52746-49-3 Usage
Description
Bathophenanthrolinedisulfonic acid disodium salt trihydrate is an organic sodium salt with the counterion 4,7-diphenyl-1,10-phenanthroline 4',4''-disulfonate. It is a slightly pinkish-beige powder that forms water-soluble complexes with palladium and has been studied for its catalytic role in the oxidation of 2-hexanol.
Uses
Used in Analytical Chemistry:
Bathophenanthrolinedisulfonic acid disodium salt trihydrate is used as a reagent for the fabrication of sensitive amperometric immunosensors, which are based on electro-click chemistry and diazonium chemistry. This application aids in the detection and quantification of specific target molecules.
Used in Biological Research:
In experimental studies, bathophenanthrolinedisulfonic acid disodium salt trihydrate is used to measure iron content and determine the solubility of iron in biological samples. This helps in understanding the correlation between iron content and the activity, preparation, and reconstitution of apoenzymes like phenylamine hydroxylase.
Used in Plant Growth:
Bathophenanthrolinedisulfonic acid disodium salt hydrate has been utilized as an iron chelator for growing plants without the need for Fe-EDTA (ethylenediaminetetraacetic acid). This application allows for the study of plant growth and development under controlled iron conditions.
Used in Environmental Science:
BATHOPHENANTHROLINEDISULFONIC ACID DISODIUM SALT TRIHYDRATE is also used in the double electrochemical covalent coupling method, which is essential for the development of sensitive and efficient environmental monitoring tools.
Biochem/physiol Actions
Bathophenanthrolinedisulfonic acid is a metal chelator. It permits iron determination to be made in aqueous medium without need for solvent extraction. It forms a complex with Fe2+ and makes it unavailable to Fe2+ transporter, thereby inhibiting the transport of iron into chloroplasts. Bathophenanthrolinedisulfonic acid is also used in the assay for determining intracellular glutathione.
Purification Methods
Dissolve crude sample in the minimum volume of water and add EtOH to precipitate the contaminants. Carefully evaporate the filtrate to obtain pure material. It forms a dark red complex with Fe2+ with 535nm ( 2.23 x 104mol-cm -1) [Imasaka et al. Anal Chim Acta 115 407 1980]. It is prepared by sulfonating bathophenanthroline with ClSO3H: to 100g of bathophenanthroline is added 0.5mL of Fe free ClSO3H and heated over a flame for 30seconds. Cool and carefully add 10mL of pure distilled H2O and warm on a water bath with stirring till all solid dissolves. A stock solution is made by diluting 3mL of this reagent to 100mL with 45% aqueous NaOAc, filter off the solid and store in a dark bottle. In this way it is stable for several months. [Landers & Bennie Am J Clinical Pathology 29 590 1958.]
Check Digit Verification of cas no
The CAS Registry Mumber 52746-49-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,2,7,4 and 6 respectively; the second part has 2 digits, 4 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 52746-49:
(7*5)+(6*2)+(5*7)+(4*4)+(3*6)+(2*4)+(1*9)=133
133 % 10 = 3
So 52746-49-3 is a valid CAS Registry Number.
InChI:InChI=1/C24H16N2O6S2.2Na.H2O/c27-33(28,29)17-5-1-15(2-6-17)19-11-13-25-23-21(19)9-10-22-20(12-14-26-24(22)23)16-3-7-18(8-4-16)34(30,31)32;;;/h1-14H,(H,27,28,29)(H,30,31,32);;;1H2/q;2*+1;/p-2
