553-24-2 Usage
Description
Basic Red 5, also known as Neutral Red, is a protonated diaminophenazine dye with the chemical formula C15H17N4Cl. It is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Basic Red 5 exhibits properties such as being a dark green powder and changing colors in response to pH levels, turning from red to yellow between pH 6.8 and 8.0.
Uses
Used in Chemical Analysis:
Basic Red 5 is used as an indicator for nitrogen determination in various chemical analyses. Its color-changing properties make it a useful tool for detecting the presence of nitrogen in samples.
Used in Histology:
In the field of histology, Basic Red 5 is a common stain used to determine cell viability. It helps in differentiating between living and dead cells, providing valuable information for researchers studying cell health and function.
Used as a Vital Stain:
Basic Red 5 can be used as a vital stain to stain living cells. Its ability to penetrate and color living cells without causing significant harm makes it a valuable tool in cell biology and microscopy.
Used in Dyes and Metabolites:
The dye properties of Basic Red 5 make it suitable for use in the production of various dyes and metabolites. Its red color with red fluorescent light brown is achieved when it is soluble in ethanol, while a cherry red color is obtained when it is soluble in water.
Used in pH Indication:
Due to its color-changing properties in response to pH levels, Basic Red 5 is used as a pH indicator. It changes from red to yellow between pH 6.8 and 8.0, making it a useful tool for monitoring and controlling pH levels in various applications.
Preparation
(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.
Biochem/physiol Actions
Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes.
Purification Methods
In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]
Check Digit Verification of cas no
The CAS Registry Mumber 553-24-2 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,5 and 3 respectively; the second part has 2 digits, 2 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 553-24:
(5*5)+(4*5)+(3*3)+(2*2)+(1*4)=62
62 % 10 = 2
So 553-24-2 is a valid CAS Registry Number.
InChI:InChI=1/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,1-3H3,(H2,16,18);1H