55894-52-5 Usage
Description
4-Nitro-3-(octanoyloxy)benzoic acid, also known as NOBA, is a chromogenic substrate specifically designed for the detection and measurement of phospholipase activity. It possesses a unique chemical structure that allows it to interact with phospholipases, such as phospholipase A2, which is commonly found in snake venom. 4-nitro-3-(octanoyloxy)benzoic acid is characterized by its ability to change color upon interaction with the enzyme, providing a visual and measurable indication of the enzyme's activity.
Uses
Used in Pharmaceutical Industry:
4-Nitro-3-(octanoyloxy)benzoic acid is used as a diagnostic tool for the detection and quantification of phospholipase A2 activity in snake venom. Its application is crucial in the development of antivenom treatments and understanding the mechanisms of venom-induced pathophysiological effects.
Used in Research and Development:
In the field of research, 4-nitro-3-(octanoyloxy)benzoic acid serves as a valuable reagent for studying the structure, function, and inhibition of phospholipases. It is particularly useful in the investigation of enzyme kinetics, enzyme inhibition, and the development of novel therapeutic agents targeting phospholipase-mediated processes.
Used in Quality Control:
4-Nitro-3-(octanoyloxy)benzoic acid is employed in the quality control of antivenom products to ensure their efficacy in neutralizing the harmful effects of phospholipase A2 present in snake venom. By measuring the absorbance at 425 nm, the compound provides a reliable and accurate method for assessing the potency of antivenom preparations.
References
1) Cho & Kezdy (1991), Chromogenic Substrates and assay of phospholipases A2; Methods Enzymol., 197 75
2) Petrovic et al. (2001), A simple assay for a human serum phospholipase A2 that is associated with high density lipoproteins; J. Lipid Res., 42 1706
3) Bonfim et al. (2008), Structural and functional characterization of myotoxin, Cr-IV 1, a phospholipase A2 D49 from the venom of the snake Calloselasma rhodostoma; Biologicals, 36 168
Check Digit Verification of cas no
The CAS Registry Mumber 55894-52-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,5,8,9 and 4 respectively; the second part has 2 digits, 5 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 55894-52:
(7*5)+(6*5)+(5*8)+(4*9)+(3*4)+(2*5)+(1*2)=165
165 % 10 = 5
So 55894-52-5 is a valid CAS Registry Number.
55894-52-5Relevant articles and documents
Evaluation of snake venom phospholipase A2: Hydrolysis of non-natural esters
Pirolla, Renan A. S.,Baldasso, Paulo A.,Marangoni, Se?rgio,Moran, Paulo J. S.,Rodrigues, Jose? Augusto R.
experimental part, p. 300 - 307 (2011/10/05)
Phospholipase A2 from the rattlesnake Crotalus durissus terrificus was employed for the first time to test its enantioseletivity on the hydrolysis of different non-natural esters. It was observed that the structure of this small enzyme is restrictive in the choice of its lipase action with non-natural substrates. Two forms of the enzyme were used; free and as its cross-linked enzyme aggregate (CLEA). With all substrates, the free enzyme showed activity similar to the CLEA preparation. The advantage of the CLEA phospholipase is the possibility to reuse it in several consecutive reactions without a decrease of activity and selectivity with good but higher yields and ee than with the free enzyme.
THE SUBSTRATE SPECIFICITY OF PHOSPHOLIPASE A2 : THE REACTION OF THE ENZYME WITH 3-OCTANOYLOXY-4-NITROBENZOIC ACID
Markowitz, Michael A.,Seykora, John T.,Kezdy, F. J.
, p. 1033 - 1038 (2007/10/02)
3-Octanoyloxy-4-nitrobenzoic acid is hydrolyzed by the phospholipase A2(D-49) from the venom of Agkistrodon piscivorus.The hydrolytic reaction is truly catalytic, requiring the presence of calcium ion (Kd=3.3 +/- 0.7mM).At pH=7.0 the value of the enzymatic rate constant is (Kcat/Km)max=175 +/- 14 M-1 sec-1, comparable to that of the enzymatic hydrolysis of 3-sn-dibutyryl lecithin.
