56599-85-0 Usage
Description
HEXADECANOIC ACID-13C16 (ALGAL SOURCE) is a long-chain fatty acid derived from algal sources, specifically labeled with 13C16 isotopes. This unique characteristic allows for its use in various analytical and research applications, particularly in the study of lipid metabolism and biosynthesis.
Uses
Used in Analytical Chemistry:
HEXADECANOIC ACID-13C16 (ALGAL SOURCE) is used as an analytical agent for the analysis of sphingolipid biosynthesis by tandem mass spectrometry. The incorporation of 13C16 isotopes in the molecule enables researchers to track and monitor the metabolic pathways and processes involving sphingolipids, providing valuable insights into their role in cellular functions and potential applications in various industries.
Used in Pharmaceutical Research:
In the pharmaceutical industry, HEXADECANOIC ACID-13C16 (ALGAL SOURCE) can be utilized as a research tool to study the effects of fatty acids on cellular processes and their potential therapeutic applications. The algal source of this compound may also offer insights into the development of novel drugs or drug delivery systems based on natural products.
Used in Nutritional Research:
HEXADECANOIC ACID-13C16 (ALGAL SOURCE) can be employed in nutritional research to investigate the role of long-chain fatty acids in human health and disease. The algal origin of this compound may provide insights into the benefits of algal-derived nutrients and their potential use in the development of functional foods or dietary supplements.
Used in Cosmetics Industry:
In the cosmetics industry, HEXADECANOIC ACID-13C16 (ALGAL SOURCE) may be used as a research tool to study the effects of fatty acids on skin health and their potential application in the development of skincare products. The algal source of this compound could also contribute to the growing interest in sustainable and eco-friendly ingredients in the cosmetics market.
Used in Environmental Research:
HEXADECANOIC ACID-13C16 (ALGAL SOURCE) can be utilized in environmental research to study the role of algal lipids in the ecosystem and their potential use in biofuel production or other sustainable applications. The unique isotopic labeling of this compound may also provide valuable information on the carbon cycling processes in aquatic environments.
Check Digit Verification of cas no
The CAS Registry Mumber 56599-85-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,6,5,9 and 9 respectively; the second part has 2 digits, 8 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 56599-85:
(7*5)+(6*6)+(5*5)+(4*9)+(3*9)+(2*8)+(1*5)=180
180 % 10 = 0
So 56599-85-0 is a valid CAS Registry Number.
56599-85-0Relevant articles and documents
Mammalian fatty acid synthase activity from crude tissue lysates tracing 13C-labeled substrates using gas chromatography-mass spectrometry
Rudolph, Michael C.,Karl Maluf,Wellberg, Elizabeth A.,Johnson, Chris A.,Murphy, Robert C.,Anderson, Steve M.
, p. 158 - 166 (2012)
Fatty acid synthase (FASN or FAS, EC 2.3.1.85) is the sole mammalian enzyme to synthesize fatty acids de novo from acetyl- and malonyl-coenzyme A (CoA) esters. This article describes a new method that directly quantifies uniformly labeled 13C16-labeled palmitate ([13C 16]palmitate) by tracing [13C2]acetyl-CoA and [13C3]malonyl-CoA using an in vitro FASN assay. This method used gas chromatography-mass spectrometry (GC-MS) to detect [ 13C16]palmitate carboxylate anions (m/z 271) of pentafluorobenzyl (PFB) derivatives and was highly sensitive at femtomole quantities. Uniformly incorporated [13C16]palmitate was the primary product of both recombinant and crude tissue lysate FASN. Quantification of FASN protein within crude tissue lysates ensured equal FASN amounts, preserved steady-state kinetics, and enabled calculation of FASN-specific activity. FASN activity determined by [13C 16]palmitate synthesis was consistent with values obtained from β-nicotinamide adenine dinucleotide 2′-phosphate (NADPH) oxidation assays. Analysis of FASN activity from tissue extracts was not hampered by contaminating enzymes or preexisting fatty acids. Crude mammary gland and liver lysates had significantly different activities at 82 and 65 nmol min -1 mg-1, respectively, suggesting that tissue-specific activity levels differ in a manner unrelated to FASN amount. GC-MS quantification of [13C16]palmitate synthesis permits sensitive evaluation of FASN activity from tissues of varied physiological states and of purified FASN activity in the presence of modifying proteins, enzymes, or drugs.
