57777-55-6

Basic information

• Product Name: 8-methylpyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione
• CAS NO: 57777-55-6
• Molecular Weight: 179.138
• Mol File: 57777-55-6.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 8-methylpyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione(CAS DataBase Reference)
• NIST Chemistry Reference: 8-methylpyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione(57777-55-6)
• EPA Substance Registry System: 8-methylpyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione(57777-55-6)

Safety Data

• Hazardous Substances Data: 57777-55-6(Hazardous Substances Data)

Upstream product

• 19359-69-4 pyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione 

Downstream Products

• 483-57-8 fervenulin 

Relevant articles and documents

Characterization of the: N -methyltransferases involved in the biosynthesis of toxoflavin, fervenulin and reumycin from Streptomyces hiroshimensis ATCC53615

Toxoflavin (1), fervenulin (2), and reumycin (3), known to be produced by plant pathogen Burkholderia glumae BGR1, are structurally related 7-azapteridine antibiotics. Previous biosynthetic studies revealed that N-methyltransferase ToxA from B. glumae BGR1 catalyzed the sequential methylation at N6 and N1 in pyrimido[5,4-e]-as-triazine-5,7(6H,8H)-dione (4) to generate 1. However, the N8 methylation of 4 in the biosynthesis of fervenulin remains unclear. To explore the N-methyltransferases required for the biosynthesis of 1 and 2, we identified and characterized the fervenulin and toxoflavin biosynthetic gene clusters in S. hiroshimensis ATCC53615. On the basis of the structures of intermediates accumulated from the four N-methyltransferase gene inactivation mutants and systematic enzymatic methylation reactions, the tailoring steps for the methylation order in the biosynthesis of 1 and 2 were proposed. The N-methylation order and routes for the biosynthesis of fervenulin and toxoflavin in S. hiroshimensis are more complex and represent an obvious departure from those in B. glumae BGR1.