57981-02-9 Usage
Description
O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA.HCl) is a white powder derivative that serves as a versatile derivatizing agent. It is widely utilized in the preparation of oximes of steroids with keto groups and as a reagent for the detection and analysis of various carbonyl-containing compounds.
Uses
Used in Steroid Preparation:
O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE is used as a reagent for the preparation of oximes of steroids with keto groups, facilitating the synthesis and modification of these biologically significant compounds.
Used in Analytical Chemistry:
O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE is used as a derivatization reagent in the determination of thromboxane B2, prostaglandins, amygdalin, and a variety of aldehydes, ketones, and acids. This application aids in the accurate identification and quantification of these compounds in various samples.
Used in Gas Chromatography and Mass Spectrometry:
O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE is used as a derivatization reagent for the detection of carbonyl-containing compounds by gas chromatography (GC), gas chromatography mass spectrometry (GC-MS), and other methods. This enhances the sensitivity and specificity of these analytical techniques, allowing for better detection and analysis of target compounds.
Used in Metabolite Analysis:
In the field of metabolomics, O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE is used for the derivatization of metabolites with carbonyl groups, followed by analysis via GC-MS. This process aids in the identification and quantification of these metabolites, contributing to a better understanding of metabolic pathways and their role in various biological processes.
Used in Aldehyde Derivatization:
O-(2,3,4,5,6-PENTAFLUOROBENZYL)HYDROXYLAMINE HYDROCHLORIDE is also used in aldehyde derivatization, followed by aldehydic hydrolysis using GC/MS. This application further expands the scope of its utility in the analysis of carbonyl-containing compounds, particularly aldehydes, in various samples.
Purification Methods
Recrystallise the salt from EtOH to form colourless leaflets. Drying the compound at high vacuum and elevated temperature will result in losses by sublimation. [Youngdale J Pharm Sci 65 625 1976, Wehner & Handke J Chromatog 177 237 1979, Nambara et al. give incorrect m as 115-116o J Chromatogr 114 81 1975.]
Check Digit Verification of cas no
The CAS Registry Mumber 57981-02-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 5,7,9,8 and 1 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 57981-02:
(7*5)+(6*7)+(5*9)+(4*8)+(3*1)+(2*0)+(1*2)=159
159 % 10 = 9
So 57981-02-9 is a valid CAS Registry Number.
InChI:InChI=1/C7H5F5NO.ClH/c8-3-2(1-14-13)4(9)6(11)7(12)5(3)10;/h1H2,13H3;1H/q+1;/p-1
