62584-33-2Relevant articles and documents
Preparation method and application of iprodione hapten and antigen
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Paragraph 0022; 0023, (2019/02/17)
The invention provides a preparation method and application of iprodione hapten and antigen. The iprodione hapten is obtained through the following steps: taking 3,5-dichlorophenyl isocyanate and ethyl glycinate hydrochloride to react to generate ethyl 2-(3-(1,5-dichlorophenylcarbamido)) acetate, hydrolyzing to obtain 2-(3-(1,5-dichlorophenylcarbamido))acetic acid, carrying out ring-forming reaction to obtain 3-(3,5-dichlorophenyl)-2,4-imidazolidindione, reacting with methyl 6-isocyanatocaproate generated by reaction of methyl 6-aminohexanoate hydrochloride and triphosgene, so as to generate methyl 6-(3-(3,5-dichlorophenyl)-2,4-dioxyiminazolealkyl-1-formylamino)caproate; finally, hydrolyzing under an acidic condition. The iprodione antigen is obtained by coupling the iprodione hapten and carrier protein. The antigen prepared by the invention has a specific iprodione antigenic determinant, so that a high-specificity iprodione monoclonal antibody is possibly screened. A generated antibody has high specificity and high sensitivity and can be used for enzyme-linked immunization and rapid determination of test paper.
Iprodione degradation by isolated soil microorganisms
Mercadier, Christine,Vega, Danielle,Bastide, Jean
, p. 207 - 215 (2007/10/03)
Three bacterial strains were isolated from soils adapted to iprodione and identified as Pseudomonas fluorescens, Pseudomonas sp. and Pseudomonas paucimobilis. The first two strains transformed iprodione to N-(3,5- dichlorophenyl)-2,4-dioxoimidazo-lidine (II) and under restrictive conditions to 3,5-dichlorophenylurea acetic acid (III); the latter subsequently degraded II to III and III to 3,5-dichloroaniline (3,5-D). We constructed bacterial combinations consisting of Pseudomonas paucimobilis plus one of the iprodione degraders and showed that these combinations transformed iprodione into 3,5- D. It is known that 3,5-D was the major metabolite found in adapted soils, suggesting that such a bacterial combination might be responsible for degrading iprodione into 3,5-D in adapted soils. Plasmids could only be isolated in Pseudomonas fluorescens but we did not investigate if one of these was involved in the ability to degrade iprodione.