6359-04-2 Usage
Description
EOSIN METHYLENE-BLUE is a histological staining agent used for the examination of blood cells. It is characterized by its brilliant blue light red color, solubility in hot water to produce a cherry red solution, and solubility in ethanol aqueous solution to yield a red solution with brown light yellow fluorescence. In concentrated sulfuric acid, it turns yellow and upon dilution, it forms a brown light yellow precipitate. When its dye aqueous solution is mixed with sodium hydroxide, it forms a dark solution with green fluorescence. It has stable properties and is slightly soluble in water.
Uses
Used in Histology:
EOSIN METHYLENE-BLUE is used as a staining agent for the histological examination of blood cells. It is often used in combination with Giemsa stain to provide a clear and detailed visualization of the cellular structures.
Used in Research and Diagnostic Laboratories:
EOSIN METHYLENE-BLUE is used as a staining agent in research and diagnostic laboratories for the identification and classification of various blood cell types. Its unique properties allow for the differentiation of different cell types based on their staining characteristics.
Used in Quality Control:
EOSIN METHYLENE-BLUE is used in the quality control of histological staining procedures to ensure the accuracy and consistency of staining results. Its light fastness and heat resistance make it a reliable choice for quality control purposes.
Used in Educational Institutions:
EOSIN METHYLENE-BLUE is used in educational institutions for teaching purposes, particularly in the fields of biology, histology, and medical research. It provides students with a practical understanding of blood cell staining and the importance of accurate staining techniques in the study of blood cells.
Preparation
Fluorescein (C.I. Acid Yellow 73, C.I. 45350) in water or ethanol solution bromide into four bromine derivatives, and then into sodium salt, then product (C.I. Acid Red 87) Metaclazepam change and translated into sylvine.
Standard
Light Fastness
Melting point
Stable
Check Digit Verification of cas no
The CAS Registry Mumber 6359-04-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 6,3,5 and 9 respectively; the second part has 2 digits, 0 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 6359-04:
(6*6)+(5*3)+(4*5)+(3*9)+(2*0)+(1*4)=102
102 % 10 = 2
So 6359-04-2 is a valid CAS Registry Number.
InChI:InChI=1/C22H12Br4O5.K/c1-2-30-22(29)10-6-4-3-5-9(10)15-11-7-13(23)18(27)16(25)20(11)31-21-12(15)8-14(24)19(28)17(21)26;/h3-8,27H,2H2,1H3;/q;+1/p-1
