65189-71-1 Usage
Description
KEMPTIDE is a basic serine-containing heptapeptide derived from a sequence of pig liver pyruvate kinase. It serves as a substrate for PKA (cyclic AMP-dependent protein kinase) and is phosphorylated and activated upon microinjection into Xenopus oocytes. The exposure of KEMPTIDE to insulin results in an increased activation of this agent, which can be inhibited by the catalytic subunit of phosphatase 2A.
Uses
Used in Research Applications:
KEMPTIDE is used as a research tool for studying the phosphorylation and activation of proteins by PKA. Its interaction with insulin and inhibition by phosphatase 2A makes it a valuable agent for understanding the mechanisms of protein kinase regulation and signal transduction pathways.
Used in Pharmaceutical Industry:
KEMPTIDE is used as a potential therapeutic agent for the development of drugs targeting protein kinases and their associated signaling pathways. Its ability to be activated by insulin and inhibited by phosphatase 2A suggests its potential use in the treatment of diseases related to insulin resistance and signal transduction disorders.
Used in Diagnostic Applications:
KEMPTIDE can be employed as a diagnostic marker for assessing the activity of PKA and other related protein kinases in various biological samples. Its phosphorylation state can provide insights into the functionality of these enzymes and their role in disease progression or response to treatment.
Biological Activity
kemptide is a synthetic heptapeptide that acts as a substrate for camp-dependent protein kinase (pk) [1].cyclic amp-dependent protein kinase is the sole intracellular acceptor for cyclic amp in all cell types. protein phosphorylation by different types of protein kinase (pk) activities comprises a form of protein modification that plays an important role in the regulation of numerous biological processes [1][2].kemptide is a novel synthetic heptapeptide phosphate acceptor that acts as a substrate for camp-dependent protein kinase. kemptide had all the properties of a cytophilic substrate and could preserving cell membrane intactness when added to cultured cells. in the presence of extracellular atp and camp, different types of intact cells catalyzed the phosphorylation of kemptide with km values of 3-4 μm. kemptide phosphorylation was influenced by pki, the inhibitory protein specific for camp-pk [1]. kemptide was usually used as a substrate for detection of activities of soluble a-kinase isolated from guinea-pig lung parenchyma and from bovine tracheal smooth muscle [2][3].
references
[1]. kübler d, pyerin w, bill o, et al. evidence for ecto-protein kinase activity that phosphorylates kemptide in a cyclic amp-dependent mode. j biol chem. 1989 aug 25;264(24):14549-55.[2]. giembycz ma, diamond j. evaluation of kemptide, a synthetic serine-containing heptapeptide, as a phosphate acceptor for the estimation of cyclic amp-dependent protein kinase activity in respiratory tissues. biochem pharmacol. 1990 jan 15;39(2):271-83.[3]. langlands jm, rodger iw. determination of soluble camp-dependent protein kinase activity in guinea-pig tracheal smooth muscle. preferential use of kemptide as a phosphorylating substrate. biochem pharmacol. 1990 apr 15;39(8):1365-74.
Check Digit Verification of cas no
The CAS Registry Mumber 65189-71-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,5,1,8 and 9 respectively; the second part has 2 digits, 7 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 65189-71:
(7*6)+(6*5)+(5*1)+(4*8)+(3*9)+(2*7)+(1*1)=151
151 % 10 = 1
So 65189-71-1 is a valid CAS Registry Number.
InChI:InChI=1/C32H61N13O9/c1-16(2)12-19(33)26(50)42-21(9-7-11-39-32(36)37)29(53)43-20(8-6-10-38-31(34)35)28(52)41-18(5)25(49)45-23(15-46)30(54)44-22(13-17(3)4)27(51)40-14-24(47)48/h16-23,46H,6-15,33H2,1-5H3,(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,44,54)(H,45,49)(H,47,48)(H4,34,35,38)(H4,36,37,39)/t18-,19-,20-,21-,22-,23-/m0/s1
