6578-06-9

Basic information

• Product Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
• Synonyms: 5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT;5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE;5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT
• CAS NO: 6578-06-9
• Molecular Formula: C₇H₉N*C₈H₆BrClNO₄P
• Molecular Weight: 433.63
• EINECS: 229-506-1
• Product Categories: Indoles;Simple Indoles;substrate
• Mol File: 6578-06-9.mol
• Article Data: 1

Chemical Properties

• Melting Point: 194-195℃
• Boiling Point: 676.2 °C at 760 mmHg
• Flash Point: 362.7 °C
• Appearance: /tablets
• Vapor Pressure: 2.65E-14mmHg at 25°C
• Storage Temp.: 2-8°C
• Solubility: DMF: 20 mg/mL
• Water Solubility: DMF: 20 mg/mL
• CAS DataBase Reference: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(6578-06-9)
• EPA Substance Registry System: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt(6578-06-9)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38-40
• Safety Statements: 26
• RIDADR: 2811
• WGK Germany: 3
• F: 3-8-10
• HazardClass: 6.1
• PackingGroup: III
• Hazardous Substances Data: 6578-06-9(Hazardous Substances Data)

Usage

Description

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, commonly known as BCIP p-toluidine, is a chemical compound that serves as a substrate for alkaline phosphatase. It is a white to light brown powder that, when reacted with alkaline phosphatase, produces a blue, water-insoluble product that can be visually detected. BCIP p-toluidine is provided in a p-toluidine form, which is soluble in dimethylformamide, and can be used to prepare a stock solution for various applications.

Uses

Used in Colorimetric Detection:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt is used as a colorimetric detection agent for alkaline phosphatase-labeled molecules. The BCIP/NBT substrate system is versatile and can be applied in various techniques such as Northern, Southern, and Western blotting, in situ hybridization, and immunohistochemistry. The reaction with alkaline phosphatase results in an insoluble NBT diformazan product, which is easily observable due to its purple color.
Used in Research and Diagnostics:
In the research and diagnostics industry, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt is used as a detection agent for the identification and analysis of specific molecules labeled with alkaline phosphatase. The visual detection of the blue, water-insoluble product allows for the assessment of molecular interactions and the presence of target molecules in various experimental setups.
Used in Pharmaceutical Development:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt may also be utilized in the pharmaceutical industry for the development of new drugs and therapies. Its role as a substrate for alkaline phosphatase can be exploited in the design of drug candidates targeting specific enzymes or pathways, potentially leading to novel treatments for various diseases.
Used in Educational Purposes:
In the field of education, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt can be employed as a teaching tool to demonstrate the principles of enzyme-substrate interactions and colorimetric detection methods. Students can observe the color change resulting from the reaction, providing a visual and engaging learning experience.
Used in Quality Control:
In the quality control industry, 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt can be used to ensure the accuracy and reliability of diagnostic tests and reagents. By incorporating BCIP p-toluidine into the testing process, manufacturers can verify the effectiveness of their products and maintain high standards of performance.

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution.

InChI:InChI=1/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

Relevant articles and documents

Azeto triazolo pyrazine β-lactam antibacterial agents

A compound of formula (I) or a salt thereof: STR1 wherein R1 is an amino group, an azido group, or an acylamino group as found in antibacterially active penicillins or cephalosporins; R2 is a hydrogen atom or a C1-4 alkyl group; R3 is a hydrogen atom, or a C1-4 alkyl group optionally substituted by a carboxyl, carboxylic ester, hydroxy, C1-4 alkyloxy, acyloxy or heterocyclylthio group; and R4 is hydrogen or a readily removable carboxyl protecting group. The compounds wherein R1 is an acylamino group are found in known antibacterially active penicillins and cephalosporins and wherein R4 is a hydrogen, a pharmaceutically acceptable salting ion or an in vivo hydrolyzable ester forming radical may be formulated in pharmaceutical compositions. Processes for the preparation of the compound (I) are also described.