66447-31-2

Basic information

• Product Name: H-LEU-AMC
• Synonyms: L-LEUCINE 7-AMIDO-4-METHYLCOUMARIN;L-LEU-7-AMINO-4-METHYLCOUMARIN;L-LEU-AMC;LEUCINE-AMC;H-LEU-AMC;(S)-2-amino-4-methyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)valeramide;L-leucine-4-methyl-7-coumarinylamidehydrochlorideforfluorescence;7-leucylamido-4-methylcoumarin
• CAS NO: 66447-31-2
• Molecular Formula: C₁₆H₂₀N₂O₃
• Molecular Weight: 288.34
• EINECS: 266-363-4
• Product Categories: Coumarins;Other enzyme inhibitors and activators;A substrate for leucine aminopeptidase and aminopeptidase M.
• Mol File: 66447-31-2.mol
• Article Data: 1

Chemical Properties

• Boiling Point: 503°Cat760mmHg
• Flash Point: 258°C
• Appearance: White to Pale Yellow/Powder
• Density: 1.208g/cm³
• Vapor Pressure: 3.03E-10mmHg at 25°C
• Refractive Index: 1.591
• Storage Temp.: 2-8°C
• PKA: 13.02±0.20(Predicted)
• CAS DataBase Reference: H-LEU-AMC(CAS DataBase Reference)
• NIST Chemistry Reference: H-LEU-AMC(66447-31-2)
• EPA Substance Registry System: H-LEU-AMC(66447-31-2)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 3-8-10
• Hazardous Substances Data: 66447-31-2(Hazardous Substances Data)

Usage

Description

H-LEU-AMC, also known as Leucine-7-amido-4-methylcoumarin, is a sensitive fluorogenic substrate commonly used in the assessment of leucine aminopeptidase (LAP) and aminopeptidase M (APM) activity. It is a white powder with unique chemical properties that allow for the detection and quantification of these enzymes.

Uses

Used in Biochemical Research:
H-LEU-AMC is used as a sensitive fluorogenic substrate for the detection and quantification of leucine aminopeptidase and aminopeptidase M activity. This application is crucial in biochemical research, as it helps scientists study the function and regulation of these enzymes, which are involved in various biological processes.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, H-LEU-AMC is used as a tool for the development and testing of new drugs targeting leucine aminopeptidase and aminopeptidase M. By monitoring the activity of these enzymes, researchers can evaluate the effectiveness of potential therapeutic agents and optimize their drug design.
Used in Diagnostic Applications:
H-LEU-AMC is also utilized in diagnostic applications, where it serves as a marker for the presence and activity of leucine aminopeptidase and aminopeptidase M. This can be particularly useful in the detection and monitoring of certain diseases or conditions where these enzymes play a significant role.
Used in Quality Control:
In the biotechnology and pharmaceutical industries, H-LEU-AMC is used as a quality control tool to ensure the purity and potency of leucine aminopeptidase and aminopeptidase M preparations. By measuring the substrate's conversion to the fluorescent product, manufacturers can verify the activity and consistency of their enzyme products.

InChI:InChI=1/C16H20N2O3/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20)/t13-/m0/s1

Downstream Products

• 1429788-64-6 C₂₂H₂₉N₃O₅S 
• 26093-31-2 7-amino-4-methylcoumarin. 
• 61-90-5 L-leucine 

Relevant articles and documents

A new fluorogenic substrate for aminopeptidase

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