66640-86-6 Usage
Description
Biotin hydrazide is a biotinyl derivative that serves as a versatile labeling agent for various biomolecules, including surface functional groups, antibodies, lectins, sugars, nucleic acids, and molecules with free carboxylic or keto groups. It is particularly useful as a probe for detecting protein carbonylation, an irreversible posttranslational modification caused by reactive oxygen species or lipid oxidation products. The reaction with biotin hydrazide is direct and does not require catalysts or reducing agents.
Uses
Used in Biochemical Research:
Biotin hydrazide is used as an aldehyde reactive biotinylation reagent for the modification of various biomolecules, enabling their detection and analysis.
Used in Glycobiology:
In the field of glycobiology, biotin hydrazide is used for the modification of alginate and as a component of glycoprotein staining solutions. It is also employed in the periodic acid-biotin-hydrazide (PABH) assay for mucins, allowing for the detection and analysis of glycoproteins and their carbohydrate moieties.
Used in Protein Analysis:
Biotin hydrazide is utilized for labeling mitochondrial proteins from non-muscle tissues, aiding in the study of protein function and localization within cellular compartments.
Used in Molecular Biology:
In molecular biology, biotin hydrazide serves as a reactive agent for labeling unpaired cytosine residues in nucleic acids, facilitating the investigation of nucleic acid structure and function.
Used in Immunology:
Biotin hydrazide is used for coupling to glycoproteins through the carbohydrate by hydrazone formation, which is particularly useful in immunological assays and the study of protein-protein interactions.
Used in Diagnostics and Therapeutics:
Biotin hydrazide is a carbohydrate reactive biotinylation reagent predominantly used for labeling monoclonal antibodies, glycoproteins, and other biologically active molecules. This labeling allows for the development of diagnostic tools and therapeutic agents targeting specific biomolecules.
Purification Methods
Wash the hydrazide with H2O, dry it, wash it with MeOH then Et2O, and dry. Recrystallise it from hot H2O (clusters of prisms) [Hofmann et al. J Biol Chem 144 513 1942]. [Beilstein 27 III/IV 7980.]
Check Digit Verification of cas no
The CAS Registry Mumber 66640-86-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,6,6,4 and 0 respectively; the second part has 2 digits, 8 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 66640-86:
(7*6)+(6*6)+(5*6)+(4*4)+(3*0)+(2*8)+(1*6)=146
146 % 10 = 6
So 66640-86-6 is a valid CAS Registry Number.
InChI:InChI=1/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1
66640-86-6Relevant articles and documents
Design, synthesis, and biological evaluation of biotinylated colchicine derivatives as potential antitumor agents
Wang, Chao,Zhang, Yujing,Wang, Zeyu,Li, Yuelin,Guan, Qi,Xing, Dongming,Zhang, Weige
, p. 411 - 420 (2021/12/24)
Chemical drug design based on the biochemical characteristics of cancer cells has become an important strategy for discovering new anti-tumour drugs to improve tumour targeting effects and reduce off-target toxicities. Colchicine is one of the most promin
KAHA ligations that form aspartyl aldehyde residues as synthetic handles for protein modification and purification
Murar, Claudia E.,Thuaud, Frdric,Bode, Jeffrey W.
supporting information, p. 18140 - 18148 (2015/03/04)
Aldehydes are widely recognized as valuable synthetic handles for the chemoselective manipulation of peptides and proteins. In this report, we show that peptides and small proteins containing the aspartic acid semialdehyde (Asa) side chain can be easily p
Mechanism-based tumor-targeting drug delivery system. Validation of efficient vitamin receptor-mediated endocytosis and drug release
Chen, Shuyi,Zhao, Xianrui,Chen, Jingyi,Chen, Jin,Kuznetsova, Larisa,Wong, Stanislaus S.,Ojima, Iwao
scheme or table, p. 979 - 987 (2011/02/22)
An efficient mechanism-based tumor-targeting drug delivery system, based on tumor-specific vitamin-receptor mediated endocytosis, has been developed. The tumor-targeting drug delivery system is a conjugate of a tumor-targeting molecule (biotin: vitamin H
