68528-80-3

Basic information

• Product Name: DISUCCINIMIDYL SUBERATE
• Synonyms: SUBERIC ACID BIS(N-HYDROXY-SUCCINIMIDE ESTER);DISUCCINIMIDYL OCTANEDIOATE;DISUCCINIMIDYL SUBERATE;DSS;n-hydroxysuccinimidesubericacidester;Bis-(N-succinimidyl)-suberate;Disuccinimidyl octanedioate, Disuccinimidyl suberate (DSS);N,N'-[Hexamethylenebis(carbonyloxy)]bissuccinimide
• CAS NO: 68528-80-3
• Molecular Formula: C₁₆H₂₀N₂O₈
• Molecular Weight: 368.34
• EINECS: 218-031-5
• Product Categories: Homobifunctional Crosslinker;homoXlink
• Mol File: 68528-80-3.mol
• Article Data: 7

Chemical Properties

• Melting Point: 169-170°C
• Boiling Point: 513.5 °C at 760 mmHg
• Flash Point: 264.4 °C
• Appearance: /powder
• Density: 1.4 g/cm³
• Storage Temp.: 2-8°C
• Solubility: chloroform: 50 mg/mL
• Water Solubility: Insoluble in water. Soluble in chloroform: 50 mg/mL.
• CAS DataBase Reference: DISUCCINIMIDYL SUBERATE(CAS DataBase Reference)
• NIST Chemistry Reference: DISUCCINIMIDYL SUBERATE(68528-80-3)
• EPA Substance Registry System: DISUCCINIMIDYL SUBERATE(68528-80-3)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• Hazardous Substances Data: 68528-80-3(Hazardous Substances Data)

Usage

Description

DSS Crosslinker is a membrane permeable, noncleavable crosslinker. The NHS ester groups are reactive toward primary amines.

Uses

Different sources of media describe the Uses of 68528-80-3 differently. You can refer to the following data:
1. Suberic acid bis(N-hydroxysuccinimide ester) has been:supplemented in pierce lysis buffer used for immunoprecipitationused to conjugate the oligonucleotide standards to gelatinused in cross-linking of ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) oligomers
2. Disuccinimidyl suberate (DSS) is a bifunctional cross-linking reagent which contains two N-hydroxysuccinimide esters that are reactive toward primary amines. DSS is membrane-permeable and is commonly used to cross-link proteins in intact cells.
3. Disuccinimidyl suberate is a homobifunctional amine-reactive crosslinker. It is usually coupled to molecules containing primary amines via amide bonds buffered at pH 7.5 (6.5-8.5). It incorporates an 8-atom spacer arm.

General Description

Suberic acid bis(N-hydroxysuccinimide ester) is a homobifunctional cross-linking reagent with amine reactivity. Typically coupled to molecules containing primary amine by amide bond buffered at pH 7.5 (6.5-8.5). Suberic acid bis(N-hydroxysuccinimide ester) incorporates an 8-atom linker.

InChI:InChI=1/C16H20N2O8/c19-11-7-8-12(20)17(11)25-15(23)5-3-1-2-4-6-16(24)26-18-13(21)9-10-14(18)22/h1-10H2

Upstream product

• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 505-48-6 octane-1,8-dioic acid 

Downstream Products

• 131865-53-7 succinimidyl 8-(4-fluorobenzylamino)-8-oxooctanoate 

Relevant articles and documents

Double strand DNA cleavage with a binuclear iron complex

Covalently linking two single strand DNA cleaving agents resulted in a new biomimetic binuclear iron complex capable of effecting oxidative double strand DNA cleavage. The Royal Society of Chemistry.

NON-HUMAN ANIMAL HAVING HUMAN CD3 GENE SUBSTITUTED FOR ENDOGENOUS CD3 GENE

The present invention provides genetically modified non-human animals which are deficient in at least one or more types of CD3 genes selected from the group consisting of endogenous CD3ε, CD3δ, and CD3γ in its genome and functionally express at least one or more types of human CD3 genes selected from the group consisting of human CD3?, CD3δ, and CD3γ. In the genetically modified non-human animals of the present invention, mature T cell differentiation and production can take place, and immunocompetent cells including T cells can exert their functions. The genetically modified non-human animals of the present invention enable efficient evaluation and screening in the development of therapeutic agents and therapeutic methods that use human CD3-mediated targeted drugs.

Preparation and properties of gelatin films incorporated with N-hydroxysuccinimide-activated end-bit binary acid

A series of novel cross-linkers, N-hydroxysuccinimide (NHS)-activated end-bit binary acid (NHS-C4, C5, C6, C8, C10, C14), were synthesised to modify gelatin films and the crosslinking effects were compared. Homogeneous films with the exception of the film crosslinked by NHS-C14 were observed and the thickness was measured using a scanning electron microscope. The section feature influenced by different film-treatment conditions was also recorded. The differential scanning calorimetry results indicated higher thermal stability. The water contact angles confirmed enhanced hydrophobicity. NHS-C6, which was used as a probe crosslinker, exhibited the best crosslinking effect that the content of the free -NH2 achieved was the lowest out of all the crosslinkers. The biodegradation results of gelatin films modified by NHS-C6 exhibited better degradation-resistance and excellent stability. In addition, the optimal experimental conditions were 45°C for 12 h when [NHS-C6]/[-NH2] = 2.5.