68792-52-9 Usage
Description
5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT, also known as 5-Methyltetrahydrofolic acid disodium salt, is a folate derivative and the sodium salt form of N-Methyltetrahydrofolic acid. It plays a crucial role in various biological processes and has potential applications in different industries due to its unique properties.
Uses
Used in Pharmaceutical Industry:
5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT is used as a pharmaceutical compound for its involvement in one-carbon metabolism, which is essential for the synthesis of nucleic acids and amino acids. It is particularly important for the proper functioning of the body's cells and has been linked to various health benefits.
Used in Nutritional Supplements:
In the nutritional supplement industry, 5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT is used as an additive to support overall health and well-being. It helps in the absorption of essential nutrients and contributes to the maintenance of a balanced diet.
Used in Research and Development:
5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT is utilized as a research compound for studying the role of folate in various biological processes and its potential applications in the development of new drugs and therapies.
Used in Cosmetics Industry:
In the cosmetics industry, 5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT is used as an ingredient in skincare products for its potential benefits in promoting skin health and maintaining a youthful appearance.
Used in Food and Beverage Industry:
5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT is employed as an additive in the food and beverage industry to enhance the nutritional value of products and support the overall health of consumers.
Biochem/physiol Actions
5-Methyl-THF acts as both cofactor and substrate in the regeneration of methionine from homocysteine through the action of 5-methyl-THF-homocysteine S-methyltransferase.
Purification Methods
First check the purity by measuring the UV at pH 7.0 (use phosphate buffer), and it should have max at 290nm and min at 245nm with a ratio of A290/A250 of 3.7. This ratio goes down to 1.3 as oxidation to the dihydro derivative occurs. The latter can be reduced back to the tetrahydro compound by reaction with 2-mercaptoethanol at room temperature. If oxidation has occurred, then the compound should be chromatographed on DEAE-cellulose (~0.9 milliequiv/g, in AcOform) in (NH4)2CO3 (1.5 M) and washed with 1M NH4OAc containing 0.01M mercaptoethanol till free from UV absorption and then washed with 0.01M mercaptoethanol. All is done in a nitrogen atmosphere. The reduced folate is then eluted with a gradient between 0.01M mercaptoethanol and 1M NH4OAc containing 0.01M mercaptoethanol, and the fractions with absorption at 290nm are collected. These are evaporated under reduced pressure at 25o, and traces of NH4OAc and H2O are removed at high vacuum/25o (~24-48hours). The residue is dissolved in the minimum volume of 0.01M mercaptoethanol, and an equivalent of NaOH is added to convert the acid to the diNa salt and evaporated to dryness at 25o/high vacuum. The pure product has max 290nm ( 32,000) in pH 7.0 buffer. [Sakami Biochemical Preparations 10 103 1963.]
Check Digit Verification of cas no
The CAS Registry Mumber 68792-52-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 6,8,7,9 and 2 respectively; the second part has 2 digits, 5 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 68792-52:
(7*6)+(6*8)+(5*7)+(4*9)+(3*2)+(2*5)+(1*2)=179
179 % 10 = 9
So 68792-52-9 is a valid CAS Registry Number.
InChI:InChI=1/C20H25N7O6.2Na/c1-27-12(9-23-16-15(27)18(31)26-20(21)25-16)8-22-11-4-2-10(3-5-11)17(30)24-13(19(32)33)6-7-14(28)29;;/h2-5,12-13,22H,6-9H2,1H3,(H,24,30)(H,28,29)(H,32,33)(H4,21,23,25,26,31);;/q;2*+1/p-2
