6976-37-0

Basic information

• Product Name: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol
• Synonyms: 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE;2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL;2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol;Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane;Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade;Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS;Bis(2-hydroxyethyl)-imino-tris-(hydroxymethyl)-methane biological buffer;bis-tris cell culture tested
• CAS NO: 6976-37-0
• Molecular Formula: C₈H₁₉NO₅
• Molecular Weight: 209.24
• EINECS: 230-237-7
• Product Categories: API intermediates;Buffer
• Mol File: 6976-37-0.mol
• Article Data: 0

Chemical Properties

• Melting Point: 104 °C(lit.)
• Boiling Point: 466.2 °C at 760 mmHg
• Flash Point: 288 °C
• Appearance: Yellow to orange/Solution
• Density: 1.346 g/cm³
• Vapor Pressure: 1.19E-10mmHg at 25°C
• Storage Temp.: Store at RT.
• Solubility: H2O: 1 M at 20 °C, clear, colorless
• PKA: 6.5(at 25℃)
• Water Solubility: soluble
• Sensitive: Air Sensitive
• BRN: 2205275
• CAS DataBase Reference: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(CAS DataBase Reference)
• NIST Chemistry Reference: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(6976-37-0)
• EPA Substance Registry System: 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol(6976-37-0)

Safety Data

• Hazard Codes: Xi,Xn
• Statements: 36/37/38-21/22-20/21/22
• Safety Statements: 26-36-36/37/39-22
• WGK Germany: 3
• F: 10-23
• TSCA: Yes
• Hazardous Substances Data: 6976-37-0(Hazardous Substances Data)

Usage

Description

2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol, also known as Bis Tris, is a versatile compound with a variety of applications in the fields of biology, chemistry, and pharmaceuticals. It is characterized by its ability to act as a biological buffer and its compatibility with a wide range of biological systems.

Uses

Used in Biological Systems:
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is used as a biological buffer for maintaining stable pH levels in various biological applications. Its pKa value of 6.36 makes it suitable for a broad range of formulations.
Used in Diagnostics:
In the diagnostics industry, 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is used as a component in diagnostic reagent formulations, contributing to the accuracy and reliability of test results.
Used in Electrophoresis:
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is used as a component in protein separation buffers for electrophoresis, aiding in the separation and analysis of proteins based on their size and charge.
Used in Protein-based Bioprocessing:
In protein-based bioprocessing, 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is used to facilitate the separation and purification of proteins, enhancing the efficiency of bioprocessing techniques.
Used in Cell Culture Media:
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is also used in the formulation of cell culture media, providing an optimal environment for the growth and maintenance of various cell types.
Used in Organic Synthesis and Pharmaceutical Intermediates:
In addition to its biological applications, 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is utilized in organic synthesis and serves as a chemical and pharmaceutical intermediate, contributing to the development of new compounds and drugs.
Used in Hemoglobin Fractionation:
2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol is used in the fractionation of hemoglobin, a process that separates different components of hemoglobin for further study and application in various fields.

Flammability and Explosibility

Notclassified

Purification Methods

Crystallise BIS-TRIS from hot 1-butanol and dry it in a vacuum at 25o. N,N-Bis-(2-hydroxyethyl)glycine (BICINE) See in “Amino Acids and Peptides”, Chapter 6.

InChI:InChI=1/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2/p+1