72669-53-5 Usage
Description
H-GLU(AMC)-OH, also known as gamma-glutamyl-7-amino-4-methylcoumarin, is a synthetic peptide substrate used in various biochemical assays. It is composed of a gamma-glutamyl group linked to a 7-amino-4-methylcoumarin (AMC) moiety. Upon enzymatic cleavage by specific enzymes, it produces a blue fluorescent solution, making it a useful tool for monitoring enzyme activity.
Uses
H-GLU(AMC)-OH is used as a fluorogenic substrate for the detection and quantification of enzyme activity in various applications.
Used in Biochemical Assays:
H-GLU(AMC)-OH is used as a substrate for aminopeptidase A or the assay of gamma-glutamyl transferase (GGT) for the detection of enzyme activity. The cleavage of the peptide bond by these enzymes releases the AMC moiety, which exhibits blue fluorescence, allowing for the measurement of enzyme activity in a sensitive and quantitative manner.
Used in Pharmaceutical Research:
H-GLU(AMC)-OH is used as a research tool in the development of new drugs targeting enzymes involved in various diseases. By monitoring the activity of these enzymes using H-GLU(AMC)-OH, researchers can evaluate the efficacy of potential drug candidates and optimize their chemical structures for better therapeutic outcomes.
Used in Quality Control:
H-GLU(AMC)-OH is used in the quality control of pharmaceutical products, ensuring that the enzymes of interest are present and active at the desired levels. This helps maintain the safety and efficacy of the products, as well as ensuring compliance with regulatory standards.
Used in Diagnostics:
H-GLU(AMC)-OH can be employed in the development of diagnostic tests for various conditions, particularly those involving the dysregulation of enzyme activity. By measuring the activity of specific enzymes using H-GLU(AMC)-OH, healthcare professionals can gain valuable insights into the underlying causes of a patient's symptoms and develop appropriate treatment plans.
Check Digit Verification of cas no
The CAS Registry Mumber 72669-53-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,2,6,6 and 9 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 72669-53:
(7*7)+(6*2)+(5*6)+(4*6)+(3*9)+(2*5)+(1*3)=155
155 % 10 = 5
So 72669-53-5 is a valid CAS Registry Number.
InChI:InChI=1/C15H16N2O5/c1-8-6-14(19)22-12-7-9(2-3-10(8)12)17-11(15(20)21)4-5-13(16)18/h2-3,6-7,11,17H,4-5H2,1H3,(H2,16,18)(H,20,21)/t11-/m0/s1
