73630-23-6

Basic information

• Product Name: QUIN 2, TETRAPOTASSIUM SALT
• Synonyms: QUIN-2;QUIN 2, TETRAPOTASSIUM SALT;2-[(2-AMINO-5-METHYLPHENOXY)METHYL]-6-METHOXY-8-AMINO-QUINOLINE-N,N,N',N'-TETRAACETIC ACID TETRAPOTASSIUM SALT;2-[[2-BIS-(CARBOXYMETHYL)AMINO-5-METHYLPHENOXY]-METHYL]-6-METHOXY-8-BIS-(CARBOXYMETHYL)AMINOQUINOLINE TETRAPOTASSIUM SALT;2-[[2-BIS(CARBOXYMETHYL)AMINO-5-METHYLPHENOXY]METHYL]-6-METHOXY-8-BIS(CARBOXYMETHYL)AMINOQUINOLINE, 4K;2-[(2-amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n',n'-tetraacetic acid tetrapotassium salt;2-[(2-Amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-N,N,Nμ,Nμ-tetraacetic acid tetrapotassium salt, 2-{[2-Bis-(carboxymethyl)amino-5-methylphenoxy]-methyl}-6-methoxy-8-bis-(carboxymethyl)aminoquinoline tetrapotassium salt;N-[2-[[8-[Bis(carboxymethyl)amino]-6-methoxy-2-quinolinyl]methoxy]-4-methylphenyl]-N-(carboxymethyl)glycine tetrapotassium salt
• CAS NO: 73630-23-6
• Molecular Formula: C26H23K4N3O10
• Molecular Weight: 693.87
• Product Categories: Quinoline
• Mol File: 73630-23-6.mol
• Article Data: 0

Chemical Properties

• Melting Point: >250℃
• Boiling Point: 831.6°C at 760 mmHg
• Flash Point: 456.7°C
• Appearance: Light yellow/powder
• Vapor Pressure: 2.58E-29mmHg at 25°C
• Storage Temp.: 2-8°C
• Solubility: H2O: soluble10mg/mL
• Water Solubility: Soluble in water at 10mg/ml
• Merck: 13,8130
• BRN: 5374262
• CAS DataBase Reference: QUIN 2, TETRAPOTASSIUM SALT(CAS DataBase Reference)
• NIST Chemistry Reference: QUIN 2, TETRAPOTASSIUM SALT(73630-23-6)
• EPA Substance Registry System: QUIN 2, TETRAPOTASSIUM SALT(73630-23-6)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• RTECS: MB8303000
• F: 3-8-10
• Hazardous Substances Data: 73630-23-6(Hazardous Substances Data)

Usage

Description

QUIN 2, Tetrapotassium Salt, also known as 2-[(2-amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n',n'-tetraacetic acid tetrapotassium salt, is a high-affinity fluorescent calcium indicator with a Kd of 115 nM for calcium. It exhibits high selectivity for calcium and is not affected by sodium gradients, membrane potential, or intracellular pH. The binding of Ca2+ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. It has excitation/emission maxima at 339 and 492 nm, respectively.

Uses

Used in Biological Research:
QUIN 2, Tetrapotassium Salt is used as a fluorescent highly selective Ca2+ indicator for the measurement of intracellular calcium signals with fluorescent indicators. It is particularly useful in studying the practical aspects of measuring cytoplasmic free calcium in biological systems.
Used in Calibration:
QUIN 2, Tetrapotassium Salt is used as a calibration substance in the measurement of cytoplasmic free calcium, helping to ensure accurate and reliable results.
Used in Buffering Intracellular Calcium Transients:
Due to its high affinity for calcium and lack of serious toxic effects at loadings of up to 2 mM, QUIN 2, Tetrapotassium Salt can be used to buffer intracellular calcium transients.
Used in conjunction with TPEN:
TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells, ensuring more accurate measurements in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) and high calcium conditions (ex. 332 nm, em. 505 nm).

Biochem/physiol Actions

Primary TargetCalcium Probes

InChI:InChI=1/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4