7411-49-6 Usage
Description
3,3',4,4'-Biphenyltetramine tetrahydrochloride is an organic compound that serves as a substrate for peroxidase and is used in the spectrophotometric determination of selenium. It is also known as 3,3'-Diaminobenzidine tetrahydrochloride and is commonly used in various applications, including the detection of hydrogen peroxide and as a substrate in immunohistochemistry and immunocytochemistry.
Uses
Used in Analytical Chemistry:
3,3',4,4'-Biphenyltetramine tetrahydrochloride is used as a reagent for the spectrophotometric determination of selenium, providing a sensitive and accurate method for measuring trace amounts of this element in various samples.
Used in Plant Biology Research:
In the detection of hydrogen peroxide in rice leaves, 3,3',4,4'-Biphenyltetramine tetrahydrochloride serves as a substrate for peroxidase, enabling researchers to study the role of hydrogen peroxide in plant processes and stress responses.
Used in Neuroscience Research:
3,3',4,4'-Biphenyltetramine tetrahydrochloride is used as a substrate for rabbit peroxidase in the immunohistochemistry of mice brain sections, allowing researchers to visualize and study specific proteins and cellular structures within the brain.
Used in Stem Cell Research:
In immunocytochemistry of muscle-derived stem cells (MDSCs), 3,3',4,4'-Biphenyltetramine tetrahydrochloride acts as a substrate for peroxidase, facilitating the detection and analysis of specific proteins and cellular components in these stem cells.
Used in Pathology and Histology:
3,3',4,4'-Biphenyltetramine tetrahydrochloride has been used for immunohistochemistry, enabling the visualization of specific antigens within tissue sections and contributing to the diagnosis and study of various diseases and conditions.
Biochem/physiol Actions
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.
Safety Profile
Suspected carcinogen withexperimental neoplastigenic and tumorigenic data. Poisonby intraperitoneal route. When heated to decomposition itemits very toxic fumes of HCl and NOx.
Purification Methods
Dissolve the salt in water and precipitate it by adding conc HCl, then drying it over solid NaOH. [Beilstein 13 IV 530.]
Check Digit Verification of cas no
The CAS Registry Mumber 7411-49-6 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,4,1 and 1 respectively; the second part has 2 digits, 4 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 7411-49:
(6*7)+(5*4)+(4*1)+(3*1)+(2*4)+(1*9)=86
86 % 10 = 6
So 7411-49-6 is a valid CAS Registry Number.
