75277-39-3 Usage
Description
HEPES sodium salt, also known as 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt, is a zwitterionic buffer that does not bind to essential metal ions such as magnesium, calcium, manganese(II), or copper(II). It is a white powder and has been classified as a Good's buffer by Dr. Norman Good and his colleagues in 1966. HEPES is most effective in the pH range of 6.8 to 8.2 and is widely used in various applications due to its chemical properties.
Uses
Used in Cell Culture Studies:
HEPES sodium salt is used as a buffer in cell culture studies to maintain a stable pH environment, which is crucial for cell growth and function.
Used in Buffer Preparation:
HEPES sodium salt is used in the preparation of HEPES buffer solution, which is essential for various biological and chemical experiments.
Used in Phosphorylation Assays:
HEPES sodium salt is used as a buffer during phosphorylation assays in permeabilized fibroblasts, ensuring a stable pH environment for accurate results.
Used in Complex Dialysis Buffer Composition:
HEPES sodium salt is used to compose a complex dialysis buffer, which is utilized in the determination of free thyroxin in serum by radioimmunoassay.
Used in Pharmaceutical and Biotechnology Industries:
HEPES sodium salt is used as a buffering agent in the pharmaceutical and biotechnology industries for various applications, including drug formulation and stability testing.
Used in Research and Development:
HEPES sodium salt is used in research and development for maintaining a stable pH environment in various biochemical and cellular assays, contributing to accurate and reliable results.
Flammability and Explosibility
Notclassified
Check Digit Verification of cas no
The CAS Registry Mumber 75277-39-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 7,5,2,7 and 7 respectively; the second part has 2 digits, 3 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 75277-39:
(7*7)+(6*5)+(5*2)+(4*7)+(3*7)+(2*3)+(1*9)=153
153 % 10 = 3
So 75277-39-3 is a valid CAS Registry Number.
75277-39-3Relevant articles and documents
Preparation of substituted alkanesulfonates from 2-hydroxyalkanesulfonates
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Page/Page column 4-6, (2008/06/13)
The present invention relates to a process for the preparation of substituted alkanesulfonates by the reaction of an aqueous metal substituted 2-hydroxyethanesulfonate with a nucleophile. 2-Alkylaminoalkanesulfonate is formed when the nucleophile is an amine. The invention also relates to the optimization of reaction conditions to produce an optimum yield.
