75350-46-8

Basic information

• Product Name: FLUORESCEIN-5-MALEIMIDE
• Synonyms: FLUORESCEIN-5-MALEIMIDE;5-Maleimido-fluorescein;N-(5-Fluoresceinyl)maleimide;1-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione;1-(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione;5-MF [Fluorescein-5-MaleiMide];5-Maleimido-fluorescein;5-MF
• CAS NO: 75350-46-8
• Molecular Formula: C₂₄H₁₃NO₇
• Molecular Weight: 427.36
• Product Categories: Fluorescent Labels and Indicators;Fluorescent Labels & Indicators;Maleimide Derivatives;MTS & Sulfhydryl Active Reagents
• Mol File: 75350-46-8.mol
• Article Data: 1

Chemical Properties

• Melting Point: >275°C (dec.)
• Boiling Point: 769.1 °C at 760 mmHg
• Flash Point: 418.9 °C
• Appearance: /
• Density: 1.73 g/cm³
• Storage Temp.: Amber Vial, -20°C Freezer, Under Inert Atmosphere
• Solubility: Acetone (Slightly), DMSO (Slightly), Methanol (Slightly)
• PKA: 9.31±0.20(Predicted)
• CAS DataBase Reference: FLUORESCEIN-5-MALEIMIDE(CAS DataBase Reference)
• NIST Chemistry Reference: FLUORESCEIN-5-MALEIMIDE(75350-46-8)
• EPA Substance Registry System: FLUORESCEIN-5-MALEIMIDE(75350-46-8)

Safety Data

• Hazard Codes: Xn
• Statements: 22
• WGK Germany: 3
• Hazardous Substances Data: 75350-46-8(Hazardous Substances Data)

Usage

Description

FLUORESCEIN-5-MALEIMIDE is a green fluorescent thiol-reactive dye that is widely used in various applications due to its high specificity and the mild conditions required for its reaction.

Uses

Used in Research and Diagnostic Applications:
FLUORESCEIN-5-MALEIMIDE is used as a labeling agent for proteins and other biomolecules in research and diagnostic applications. Its thiol-reactive nature allows for the selective modification of cysteine residues, enabling the study of protein structure, function, and interactions.
Used in Cell Biology:
In cell biology, FLUORESCEIN-5-MALEIMIDE is used as a fluorescent probe for monitoring cellular processes and detecting changes in cellular redox states. Its green fluorescence allows for the visualization of cellular structures and the tracking of protein dynamics within living cells.
Used in Drug Delivery Systems:
FLUORESCEIN-5-MALEIMIDE can be used as a fluorescent marker in drug delivery systems, allowing for the tracking and monitoring of drug distribution, release, and uptake in biological systems. This can be particularly useful in the development of targeted drug delivery systems and in studying the pharmacokinetics and pharmacodynamics of therapeutic agents.
Used in Environmental Monitoring:
In environmental monitoring, FLUORESCEIN-5-MALEIMIDE can be employed as a tracer to study the behavior and fate of pollutants in the environment. Its green fluorescence enables the detection and quantification of contaminants in water, soil, and air samples, aiding in the assessment of environmental pollution and the development of remediation strategies.
Used in Chemical Sensing:
FLUORESCEIN-5-MALEIMIDE can be utilized as a chemical sensor for the detection of various analytes, such as metal ions, small molecules, and gases. Its fluorescence properties can be modulated upon interaction with target molecules, allowing for the development of highly sensitive and selective sensing platforms.
Used in Material Science:
In material science, FLUORESCEIN-5-MALEIMIDE can be incorporated into the design of advanced materials, such as polymers, nanoparticles, and hydrogels, to impart fluorescent properties. These materials can be used in a range of applications, including sensors, imaging agents, and stimuli-responsive systems.

Upstream product

• 3326-34-9 fluoresceinamine 

Relevant articles and documents

Efficient synthesis of fluorophore-linked maleimide derivatives

Lewis acid and hexamethyldisilazane promoted efficient synthesis of various fluorophore-linked maleimide derivatives from maleic anhydride and amines is described.