754201-87-1Relevant articles and documents
Synthesis and characterisation of novel chromogenic substrates for human pancreatic α-amylase
Damager, Iben,Numao, Shin,Chen, Hongming,Brayer, Gary D.,Withers, Stephen G.
, p. 1727 - 1737 (2007/10/03)
Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic α-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of α-amylases, and are useful in rapidly screening for novel α-amylase inhibitors and for subsequent kinetic characterisation.