79580-69-1Relevant articles and documents
Lipase PS (Pseudomonas cepacia) mediated resolution of γ-substituted γ-((acetyloxy)methyl)-γ-butyrolactones: Complete stereochemical reversion by substituents
Ha, Hyun-Joon,Yoon, Kyung-Noh,Lee, Sang-Youn,Park, Young-Soo,Lim, Moon-Soo,Yim, Yoon-Gil
, p. 8062 - 8066 (1998)
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Adrenaline profiling of lipases and esterases with 1,2-diol and carbohydrate acetates
Wahler, Denis,Boujard, Olivier,Lefèvre, Fabrice,Reymond, Jean-Louis
, p. 703 - 710 (2007/10/03)
The adrenaline test for enzymes is a general back-titration procedure to detect 1,2-diols, 1,2-aminoalcohols and α-hydroxyketones reaction products of enzyme catalysis by colorimetry. The method was used to profile a series of esterases and lipases for their esterolytic activity on a series of carbohydrate and polyol acetates. Substrates were prepared by peracetylation and used for parallel microtiter-plate analysis of enzyme activities. This method can be used to achieve a rapid and automated characterization of a set of enzymes during HTS screening.