80293-60-3Relevant articles and documents
Conjugation with betaine: A facile and effective approach to significant improvement of gene delivery properties of PEI
Sun, Jun,Zeng, Fang,Jian, Haoliang,Wu, Shuizhu
, p. 728 - 736 (2013)
Herein, we developed a new gene delivery vector by grafting a betaine monomer (N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate, DMAAPS) onto 25 KDa polyethylenimine (PEI 25K) via the Michael addition reaction. The graft ratio for betaine on PEI polymer could be readily controlled, and in this study three PEI-betaine conjugates PEI-DMAAPS23%, PEI-DMAAPS55%, and PEI-DMAAPS95% were prepared with their graft ratios of 23, 55, and 95%, respectively. The PEI-betaine conjugates exhibited much lower protein adsorption and cytotoxicities compared with PEI 25K, and they also showed little or no hemolytic effect. Moreover, the PEI-betaine conjugates display satisfactory DNA condensation capability; and in the absence and presence of serum, PEI-DMAAPS23%/pEGFP and PEI-DMAAPS55%/pEGFP complexes exhibited remarkable gene transfection efficiencies determined by flow cytometry, which are in general several times higher than that of PEI 25K. With these favorable properties, the PEI-betaine conjugates hold great potential for use as efficient gene delivery vectors. This study suggests that the betaine monomer may serve as a biocompatible modifying agent and this facile strategy may provide a facile and effective way for constructing some other biocompatible materials.
Thermosensitive properties of semi-IPN gel composed of amphiphilic gel and zwitterionic thermosensitive polymer in buffer solutions containing high concentration salt
Takahashi, Atsushi,Hamai, Kenta,Okada, Yuko,Sakohara, Shuji
, p. 3791 - 3799 (2011)
In this study, a semi-interpenetrating polymer network (semi-IPN) gel, which consists of an amphiphilic N,N-dimethylacrylamide-co-N-isopropylacrylamide (DMAA-co-NIPAM) gel and an interpenetrating zwitterionic thermosensitive poly(N-isopropylacrylamide-co-N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate) (poly(NIPAM-co-DMAAPS)) was prepared. The thermosensitive behavior of the semi-IPN gel was investigated in a buffer solution composed of a relatively high concentration of sodium chloride and sodium citrate as salts, and sodium dodecyl sulfate as a surfactant, which are generally used as a buffer solution in biochips. At low temperatures, the semi-IPN gel in the buffer solution was absolutely transparent; however, when the gel was heated, the gel became milky white or opaque without a large change in the gel size. The network of the transparent gel is homogeneous, whereas that of the opaque gel consists of coarse and dense parts. Such a structural change in the gel network was confirmed by the temperature dependence of the permeability of the buffer solution through the semi-IPN gel membrane. The permeability increased drastically when the gel became opaque because of heating.
Investigation of ion adsorption properties of sulfobetaine gel and relationship with its swelling behavior
Ningrum, Eva Oktavia,Murakami, Yukiko,Ohfuka, Yasuhiro,Gotoh, Takehiko,Sakohara, Shuji
, p. 5189 - 5197 (2014)
The adsorption of cations and anions in nitrate solutions on N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate (DMAAPS) gels prepared using various cross-linker and monomer concentrations was investigated. The influence of the temperature and nitrate concentration on the adsorption properties of the gel was evaluated, demonstrating simultaneous adsorption of cations and anions. The amount of Zn2+adsorbed on the gel in Zn(NO3)2solution increased as the cross-linker and monomer concentrations used in the gel preparation increased. For the gel prepared using a higher cross-linker or monomer concentration, elevation of the temperature did not induce any significant change in the amount of Zn2+adsorbed on the gel. Furthermore, for the gel prepared using a lower cross-linker or monomer concentration, the amount of Zn2+adsorbed on the gel decreased significantly as the temperature increased. In addition, an interesting correlation between the degree of swelling of the gel and the amount of Zn2+adsorbed on the gel was found. As the degree of swelling decreased, the adsorption amount increased to eventually achieve a constant value.
