8063-24-9

Basic information

• Product Name: Acriflavine hydrochloride
• Synonyms: LISTERIA SELECTIVE SUPPLEMENT UVM-II;LISTERIA PALCAM SELECTIVE SUPPLEMENT;EUFLAVINE;EUFLAVIN HYDROCHLORIDE;CI 46000;3,6-DIAMINO-10-METHYLACRIDINIUM CHLORIDE HYDROCHLORIDE;ACRIFLAVINE HCL;ACRIFLAVINE HYDROCHLORIDE
• CAS NO: 8063-24-9
• Molecular Formula: C27H28Cl4N6
• Molecular Weight: 578.36
• EINECS: 1312995-182-4
• Mol File: 8063-24-9.mol
• Article Data: 0

Chemical Properties

• Melting Point: 260 °C
• Appearance: Red-brown/Powder
• Storage Temp.: +2°C TO
• Solubility: H2O: soluble1mg/mL
• Water Solubility: Soluble in water, dimethyl sulfoxide and methanol.
• Stability: Stable. Incompatible with strong oxidizing agents.
• Merck: 123
• BRN: 6265525
• CAS DataBase Reference: Acriflavine hydrochloride(CAS DataBase Reference)
• NIST Chemistry Reference: Acriflavine hydrochloride(8063-24-9)
• EPA Substance Registry System: Acriflavine hydrochloride(8063-24-9)

Safety Data

• Hazard Codes: N-Xn,N,Xn
• Statements: 51/53-41-22-20/21/22
• Safety Statements: 61-39-29-26-36
• RIDADR: UN 3077 9/PG 3
• WGK Germany: 2
• HazardClass: 9
• PackingGroup: III
• Hazardous Substances Data: 8063-24-9(Hazardous Substances Data)

Usage

Description

Acriflavine hydrochloride is a synthetic, acridine-based dye with antiseptic properties. It is known for its broad-spectrum antimicrobial activity and is commonly used as a topical agent for disinfection and treatment of various infections.
Used in Pharmaceutical Industry:
Acriflavine hydrochloride is used as a topical antiseptic agent for the treatment of external fungal infections. Its antimicrobial properties make it effective against a wide range of microorganisms, including bacteria, fungi, and viruses.
Used in Research Applications:
Acriflavine hydrochloride is used as an intercalating dye in research settings, particularly for the dual fluorescence analysis of cellular DNA and protein simultaneously. Its ability to bind to nucleic acids allows for the visualization and study of cellular components.
Additionally, Acriflavine hydrochloride has been reported to inhibit mitochondriogenesis, making it a useful tool in studying mitochondrial function and biogenesis in various cell types.

Purification Methods

Purify it by dissolving in 50 parts of H2O, shaking with a small excess of freshly precipitated and washed Ag2O. The mixture is set aside overnight at 0o and filtered. The cake is not washed. The pH of the filtrate is adjusted to 7.0 with HCl and evaporated to dryness. The residue is then crystallised twice from MeOH, twice from H2O and dried at 120o. at 452nm has a log value of 4.67. Itis ared max powder which readily absorbs H2O. The solubility is increased in the presence of proflavin. The dihydrochloride is a deep red crystalline powder. It is available as a mixture of 3,6-diaminoacridinium chloride (35%) and its 10-metho-chloride (65%). [See Albert, The Acridines Arnold Press p 346 1966, Benda Chem Ber 45 1787 1912]. [Beilstein 23 I 650.]

InChI:InChI=1/C14H13N3.C13H11N3.2ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;2*1H