80750-24-9

Basic information

• Product Name: DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER
• Synonyms: DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER;N-Hydroxysuccinimido dethiobiotinate;(4R,5S)-5-Methyl-2-oxo-4-imidazolidinehexanoic acid 2,5-dioxo-1-pyrrolidinyl ester;Desthiobiotin N-hydroxysuccinimide ester
• CAS NO: 80750-24-9
• Molecular Formula: C14H21N3O5
• Molecular Weight: 311.33
• Mol File: 80750-24-9.mol
• Article Data: 4

Chemical Properties

• Appearance: /
• CAS DataBase Reference: DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER(CAS DataBase Reference)
• NIST Chemistry Reference: DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER(80750-24-9)
• EPA Substance Registry System: DESTHIOBIOTIN N-HYDROXYSUCCINIMIDYL ESTER(80750-24-9)

Safety Data

• Hazardous Substances Data: 80750-24-9(Hazardous Substances Data)

Usage

Description

Desthiobiotin N-Hydroxysuccinimide Ester is a chemical compound that serves as an intermediate in the synthesis of various biotin-related probes and conjugates. It is characterized by its ability to react with amines to form stable amide bonds, making it a versatile reagent in the development of biotin-based assays and detection methods.

Uses

Used in Biochemical Research:
Desthiobiotin N-Hydroxysuccinimide Ester is used as a reactant for the preparation of fluorescein-labeled Dethiobiotin (D297663) probe, which is specifically designed for continuous fluorescence displacement assay of BioA enzyme activity. BioA is an enzyme that plays a crucial role in the biosynthesis of Biotin (B389040), an essential vitamin for various metabolic processes in living organisms.
This application is particularly important in the study of biotin biosynthesis pathways, enzyme mechanisms, and the development of novel assays for monitoring enzyme activity. By using Desthiobiotin N-Hydroxysuccinimide Ester in the synthesis of the probe, researchers can gain valuable insights into the regulation and function of BioA enzyme, as well as the broader context of biotin metabolism.

Upstream product

• 15720-25-9 Desthiobiotin 
• 68-04-2 sodium citrate 
• 6066-82-6 1-hydroxy-pyrrolidine-2,5-dione 
• 533-48-2 desthiobiotin 

Downstream Products

• 1306615-47-3 C₁₈H₃₄N₆O₅ 

Relevant articles and documents

Visible detection of biotin by thin-film interference: Thickness control through exchange reaction of biotin/dethiobiotin-avidin bonding

This work presents a unique method for the visible detection of site-specific molecular interactions between proteins and small molecules. In this method, a silica thin-film, which shows thin-film interference, was used as a substrate. On its surface, protein multilayers made up of avidin- and dethiobiotin-labeled bovine serum albumin (BSA) were constructed via the avidin-dethiobiotin bond. Film construction behaviors were confirmed visibly owing to the uniform change of the interference color. This multilayer was able to disassemble by biotin addition, taking advantage of the higher association constant of avidin-biotin bonding. Disassembling of the multilayer was also confirmable as a drastic color change seen by the naked eye. The Royal Society of Chemistry.

COMPACT HYDROXAMATE-BASED AFFINITY TAGS FOR ARTIFICIALLY TAGGING BIOLOGICAL MACROMOLECULES

Methods for purifying biological macromolecules are provided. Aspects of the subject methods include contacting the biological macromolecule with an exemplary hydroxamate affinity tag to produce a tagged moiety followed by purification of the tagged moiety by immobilized metal affinity chromatography (IMAC). Also provided are kits comprising an exemplary subject hydroxamate affinity tag, an IMAC resin and a metal ion configured for loading onto the resin, wherein the metal ion is capable of binding to a compound containing the hydroxamate affinity tag.