82-94-0 Usage
Description
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.
Uses
Used in Microscopy:
Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronin Y dye for one stage DNA (green) and RNA (red) staining method.
Used in Counterstaining:
Methyl Green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.
Used in Enzymatic Activity and Mitochondrial Staining:
Methyl Green is used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann.
Preparation
chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction
Standard( Cotton )
Light Fastness
Fading
Stain
C
1
Check Digit Verification of cas no
The CAS Registry Mumber 82-94-0 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 8 and 2 respectively; the second part has 2 digits, 9 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 82-94:
(4*8)+(3*2)+(2*9)+(1*4)=60
60 % 10 = 0
So 82-94-0 is a valid CAS Registry Number.
InChI:InChI=1/C26H33N3.2ClH/c1-27(2)23-14-8-20(9-15-23)26(21-10-16-24(17-11-21)28(3)4)22-12-18-25(19-13-22)29(5,6)7;;/h8-19H,1-7H3;2*1H/q+2;;/p-2
