871664-01-6Relevant articles and documents
Template-directed DNA photoligation via α-5-cyanovinyldeoxyuridine
Ogino, Masayuki,Yoshimura, Yoshinaga,Nakazawa, Akio,Saito, Isao,Fujimoto, Kenzo
, p. 2853 - 2856 (2007/10/03)
(Chemical Equation Presented) We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using α-5- cyanovinyldeoxyuridine (αcU). An efficient photoligation was produced by photoirradiation of an ODN containing thymine at the 5′ end with an ODN containing thymine at the 5′ end in the presence of a template ODN. This photoligation method is a new and efficient way to synthesize branched ODNs.