88793-79-7 Usage
Description
N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT, also known as Chromozym PL, is a synthetic peptide substrate used in various biochemical assays to evaluate enzymatic activity. It is composed of a series of amino acids, including glycine, proline, lysine, and p-nitroaniline, with a tosyl group attached to the N-terminus and an acetate salt group at the C-terminus. N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT is particularly useful in the study of proteolytic enzymes, such as serine proteases and plasmin, due to its ability to produce a colorimetric change upon cleavage, allowing for easy quantification of enzyme activity.
Uses
Used in Biochemical Research:
N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT is used as a substrate for examining the amidolytic activity of enzymes such as aprE2 and aprE5-41. This allows researchers to study the catalytic properties and mechanisms of these enzymes, as well as their potential roles in various biological processes.
Used in Enzyme Assays:
N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT is used as a substrate for serine protease and fibrinogenolytic assays. These assays are essential for understanding the function and regulation of serine proteases, which are involved in numerous physiological processes, including blood clotting, inflammation, and tissue remodeling.
Used in Plasmin Assays:
N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT is used as a substrate for plasmin assays. Plasmin is a key enzyme in the fibrinolytic system, responsible for breaking down fibrin clots in the blood. By using this substrate, researchers can evaluate the activity of plasmin and its regulators, which can provide insights into the mechanisms of blood clot dissolution and potential therapeutic targets for conditions such as thrombosis.
Used in Determining Plasmin Enzymatic Activity:
Chromozym PL, the commercial form of N-P-TOSYL-GLY-PRO-LYS P-NITROANILIDE ACETATE SALT, is used to determine the plasmin enzymatic activity using human umbilical vein endothelial cells (HUVEC) cell extracts. This application is crucial for studying the role of plasmin in vascular health and disease, as well as for developing potential therapeutic agents that modulate plasmin activity.
Used in Proteolytic Activity Assessment:
Chromozym PL is used as a chromogenic substrate for proteolytic activity in murine lung homogenates. This allows researchers to investigate the role of proteolytic enzymes in lung physiology and pathology, such as in the context of inflammatory processes and tissue remodeling.
Used in Plasminogen Activation Assays:
Chromozym PL is used as a substrate of plasmin in plasminogen activation assays. These assays are essential for understanding the regulation of the fibrinolytic system and the conversion of plasminogen to active plasmin, which can provide insights into the development of therapeutic agents for conditions such as thrombotic disorders and inflammatory diseases.
Biochem/physiol Actions
N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt is a chromogenic substrate for plasmin assay, serine protease enzyme activity and fibrinogenolytic assays.
Check Digit Verification of cas no
The CAS Registry Mumber 88793-79-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 8,8,7,9 and 3 respectively; the second part has 2 digits, 7 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 88793-79:
(7*8)+(6*8)+(5*7)+(4*9)+(3*3)+(2*7)+(1*9)=207
207 % 10 = 7
So 88793-79-7 is a valid CAS Registry Number.
InChI:InChI=1/C26H34N6O7S.C2H4O2/c1-18-7-13-21(14-8-18)40(38,39)31(19-9-11-20(12-10-19)32(36)37)17-24(33)30-16-4-6-23(30)26(35)29-22(25(28)34)5-2-3-15-27;1-2(3)4/h7-14,22-23H,2-6,15-17,27H2,1H3,(H2,28,34)(H,29,35);1H3,(H,3,4)/t22-,23-;/m0./s1
