89-36-1 Usage
Description
3-Methyl-1-(4-sulfophenyl)-2-pyrazolin-5-one is an organic compound that features a pyrazolin-5-one core with a methyl and a sulfophenyl group attached. It is known for its potential applications in various fields, particularly in the development of reagents for chemical analysis and biomedical research.
Uses
Used in Chemical Analysis:
3-Methyl-1-(4-sulfophenyl)-2-pyrazolin-5-one is used as a derivatization reagent for the pre-column labeling of carbohydrates. This application aids in the detection and analysis of carbohydrates in complex biological samples, enhancing the sensitivity and accuracy of analytical techniques.
Used in Carbohydrate Analysis:
In carbohydrate analysis, 3-Methyl-1-(4-sulfophenyl)-2-pyrazolin-5-one serves as a derivatization agent for the determination of oligosides, mannose, and galactose. These sugars are obtained through the degradation of galactomannans, and the reagent helps in their identification and quantification, which is crucial for understanding the structure and function of these complex carbohydrates.
Used in Cancer Therapy Research:
3-Methyl-1-(4-sulfophenyl)-2-pyrazolin-5-one acts as a reagent for identifying sulfonic acids as potential ecto-5'-nucleotidase inhibitors. The inhibition of ecto-5'-nucleotidase is considered a promising approach in cancer therapy, as it can modulate the activity of this enzyme, which is implicated in various cancer-related processes. This application highlights the compound's utility in the development of novel therapeutic strategies against cancer.
Check Digit Verification of cas no
The CAS Registry Mumber 89-36-1 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 8 and 9 respectively; the second part has 2 digits, 3 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 89-36:
(4*8)+(3*9)+(2*3)+(1*6)=71
71 % 10 = 1
So 89-36-1 is a valid CAS Registry Number.
InChI:InChI=1/C10H10N2O4S/c1-7-6-10(13)12(11-7)8-2-4-9(5-3-8)17(14,15)16/h2-5H,6H2,1H3,(H,14,15,16)/p-1
89-36-1Relevant articles and documents
Virtual ligand screening of the p300/CBP histone acetyltransferase: Identification of a selective small molecule inhibitor
Bowers, Erin M.,Yan, Gai,Mukherjee, Chandrani,Orry, Andrew,Wang, Ling,Holbert, Marc A.,Crump, Nicholas T.,Hazzalin, Catherine A.,Liszczak, Glen,Yuan, Hua,Larocca, Cecilia,Saldanha, S. Adrian,Abagyan, Ruben,Sun, Yan,Meyers, David J.,Marmorstein, Ronen,Mahadevan, Louis C.,Alani, Rhoda M.,Cole, Philip A.
scheme or table, p. 471 - 482 (2011/08/06)
The histone acetyltransferase (HAT) p300/CBP is a transcriptional coactivator implicated in many gene regulatory pathways and protein acetylation events. Although p300 inhibitors have been reported, a potent, selective, and readily available active-sitedirected small molecule inhibitor is not yet known. Here we use a structure-based, in silico screening approach to identify a commercially available pyrazolone- containing small molecule p300 HAT inhibitor, C646. C646 is a competitive p300 inhibitor with a Ki of 400 nM and is selective versus other acetyltransferases. Studies on site-directed p300 HAT mutants and synthetic modifications of C646 confirm the importance of predicted interactions in conferring potency. Inhibition of histone acetylation and cell growth by C646 in cells validate its utility as a pharmacologic probe and suggest that p300/CBP HAT is a worthy anticancer target.
