9001-86-9

Basic information

• Product Name: EC 3.1.4.3
• Synonyms: lipophosphodiesterase;phosphotidasec;PHOSPHATIDYLCHOLINE CHOLINEPHOSPHOHYDROLASE;PHOSPHATIDYLCHOLINE CHOLINE PHOSPHOHYDROLASE TYPE I;PHOSPHATIDYLCHOLINE CHOLINE-PHOSPHOHYDROLASE TYPE IX;PHOSPHATIDYLCHOLINE CHOLINE-PHOSPHOHYDROLASE TYPE XIV;PHOSPHOLIPASE C;PHOSPHOLIPASE C, BACILLUS CEREUS
• CAS NO: 9001-86-9
• Molecular Weight: 0
• EINECS: 232-638-2
• Mol File: 9001-86-9.mol
• Article Data: 0

Chemical Properties

• Appearance: slightly beige/powder
• Storage Temp.: −20°C
• Solubility: H2O: soluble1.0mg/mL, faintly hazy to hazy
• CAS DataBase Reference: EC 3.1.4.3(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.1.4.3(9001-86-9)
• EPA Substance Registry System: EC 3.1.4.3(9001-86-9)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• RTECS: SZ6116000
• F: 3-10
• Hazardous Substances Data: 9001-86-9(Hazardous Substances Data)

Usage

Description

EC 3.1.4.3, also known as Phospholipase C, is an enzyme that catalyzes the hydrolysis of inositol-containing phospholipids, playing a crucial role in various cellular processes, including signal transduction and membrane trafficking. It has been widely studied and applied in different fields due to its diverse functions and potential applications.

Uses

Used in Biomedical Research:
EC 3.1.4.3 is used as a research tool for studying adrenoceptor-mediated transmembrane signaling, which is essential for understanding various physiological processes and the development of targeted therapies.
Used in Biochemistry:
The enzyme is used to degrade inositol-containing phospholipids, providing insights into the molecular mechanisms underlying cellular functions and contributing to the advancement of biochemistry.
Used in Molecular Biology:
EC 3.1.4.3 is used to study the activation of the 9E3 gene, which may have implications in gene regulation and the development of novel therapeutic strategies.
Used in Diagnostics:
Phospholipase C from Clostridium perfringens is used as a positive control in sandwich enzyme-linked immunosorbent assay (ELISA) and ELISA, ensuring the accuracy and reliability of diagnostic tests.
Used in Parasitology:
The enzyme from Sigma has been used in the digestion of the phosphatidylserine fraction of Schistosoma mansoni adult worms, aiding in the study of parasitic diseases and their treatment.
Used in Environmental Research:
This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research, contributing to the development of sustainable and eco-friendly energy sources.

Biochem/physiol Actions

PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.