9012-90-2

Basic information

• Product Name: DNA polymerase
• Synonyms: DNA POLYMERASE;DNA POLYMERASE 1, KLENOV FRAGMENT;DNA POLYMERASE 1, KLENOW FRAGMENT;DNA POLYMERASE 1, LARGE FRAGMENT;DNA POLYMERASE I;DNA POLYMERASE I, KLENOW;DNA POLYMERASE I (KLENOW FRAGMENT);DNA POLYMERASE I, LARGE FRAGMENT
• CAS NO: 9012-90-2
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-741-2
• Product Categories: 2.7.x.x Phosphorus containing groups;Nucleic Acid Labeling Enzymes and ReagentsApplication Index;PCRMolecular Biology;Routine AmplificationPlant Molecular Biology;Routine PCR AmplificationEnzyme Class Index;2.x.x.x Transferases;Molecular Biology;Molecular Biology Tested;Nucleic Acid Amplification;Nucleic Acid Detection and Hybridization;PCR/Amplification;Modifying EnzymesMolecular Biology;Nucleic Acid Labeling Enzymes and Reagents;Modifying EnzymesApplication Index;Molecular Biology Enzymes
• Mol File: 9012-90-2.mol
• Article Data: 0

Chemical Properties

• Appearance: colorless/buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: DNA polymerase(CAS DataBase Reference)
• NIST Chemistry Reference: DNA polymerase(9012-90-2)
• EPA Substance Registry System: DNA polymerase(9012-90-2)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38-43
• Safety Statements: 26-36-24/25-36/37
• WGK Germany: 3
• F: 10
• TSCA: Yes
• Hazardous Substances Data: 9012-90-2(Hazardous Substances Data)

Usage

Description

DNA polymerase is an enzyme that plays a crucial role in the replication and synthesis of DNA molecules. It is responsible for adding nucleotides to the growing DNA strand during replication, ensuring the accurate transmission of genetic information. DNA polymerases are found in all living organisms, from bacteria to humans, and are essential for maintaining the integrity of the genetic code.

Uses

Used in Molecular Biology Research:
DNA polymerase is used as a key component in various molecular biology techniques for DNA amplification, sequencing, and analysis. It is employed in processes such as DNA extraction, genotyping, and gene expression studies to investigate the constitutive production of specific proteins and other molecules.
Used in Polymerase Chain Reaction (PCR):
DNA polymerase is used as a critical enzyme in the PCR process, which allows for the amplification of specific DNA sequences. This technique is widely used in research, diagnostics, and forensics to detect and analyze genetic material.
Used in Reverse Transcription-Polymerase Chain Reaction (RT-PCR):
DNA polymerase is also used in RT-PCR, a technique that converts RNA into complementary DNA (cDNA) and then amplifies it using PCR. This method is useful for studying gene expression and analyzing RNA levels in various biological samples.
Used in DNA Sequencing:
DNA polymerase is employed in DNA sequencing methods, such as Sanger sequencing, to determine the order of nucleotides in a DNA molecule. This information is vital for understanding genetic variations and their implications in health and disease.
Used in Genetic Engineering:
DNA polymerase is used in various genetic engineering applications, such as cloning, site-directed mutagenesis, and the construction of recombinant DNA molecules. These techniques are essential for developing new therapies, improving crop yields, and creating genetically modified organisms.
Used in Diagnostics:
DNA polymerase is used in diagnostic tests to detect the presence of specific genetic markers or pathogens, such as viruses or bacteria. This helps in the early diagnosis and treatment of various diseases, as well as in monitoring the spread of infectious agents.
Used in Forensic Science:
DNA polymerase is employed in forensic science for DNA profiling and identification. It is used to analyze DNA samples from crime scenes, allowing for the comparison of genetic material and the identification of suspects or victims.
Used in Preamplification Mix for Amplified Fragment Length Polymorphism (AFLP) Analysis:
DNA polymerase is used as a component of the preamplification mix for PCR in AFLP analysis, a technique used to study genetic variation and relationships among different organisms, such as Fusarium oxysporum sp. isolates.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.