9013-18-7

Basic information

• Product Name: ACYL-COENZYME A SYNTHETASE
• Synonyms: EC 6.2.1.3;ACID: COA LIGASE (AMP-FORMING);ACYL-COENZYME A SYNTHETASE;Synthetase, acyl coenzyme A;ACYL-COENZYME A SYNTHETASE FROM PSEUDO-M ONAS SP., ~2.5U/MG;acyl-coenzyme a synthetase from pseudomonas sp.;Acyl-coenzyme A Synthetase [>=8 units/mg];Acyl-CoA Synthetase
• CAS NO: 9013-18-7
• Molecular Weight: 0
• EINECS: 232-747-5
• Mol File: 9013-18-7.mol
• Article Data: 0

Chemical Properties

• Appearance: white/powder
• Storage Temp.: −20°C
• CAS DataBase Reference: ACYL-COENZYME A SYNTHETASE(CAS DataBase Reference)
• NIST Chemistry Reference: ACYL-COENZYME A SYNTHETASE(9013-18-7)
• EPA Substance Registry System: ACYL-COENZYME A SYNTHETASE(9013-18-7)

Safety Data

• Safety Statements: 24/25
• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9013-18-7(Hazardous Substances Data)

Usage

Chemical Properties

White powder

Uses

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)in in vitro fatty acylation assaysin the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA

General Description

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Biochem/physiol Actions

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .

Purification Methods

Purify the synthase by extraction with sucrose/HCO3 buffer, protamine sulfate precipitation, (NH4)2SO4 (66-65%) fractination (pH 4.35) and a second (NH4)2SO4 (35-60%) fractionation (pH 4.35). It has Km 0.15mM (Vrel 1.0) for octanoate and 0.41mM (Vrel 2.37) for heptanoate. The Km for ATP is 0.5mM, all at pH 9.0 in ethylene glycol buffer at 38o. [Jencks et al. J Biol Chem 204 453 1953, Methods Enzymol 5 467 1962.]