90217-02-0 Usage
Description
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.
Uses
Different sources of media describe the Uses of 90217-02-0 differently. You can refer to the following data:
1. Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity.
2. 5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells.
Check Digit Verification of cas no
The CAS Registry Mumber 90217-02-0 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,0,2,1 and 7 respectively; the second part has 2 digits, 0 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 90217-02:
(7*9)+(6*0)+(5*2)+(4*1)+(3*7)+(2*0)+(1*2)=100
100 % 10 = 0
So 90217-02-0 is a valid CAS Registry Number.
InChI:InChI=1/C16H14N5.ClH/c1-12-3-7-14(8-4-12)20-18-16(11-17)19-21(20)15-9-5-13(2)6-10-15;/h3-10H,1-2H3;1H/q+1;/p-1
