9025-65-4

Basic information

• Product Name: MUNG BEAN NUCLEASE
• Synonyms: S1 NUCLEASE;NUCLEASE, MUNG BEAN;NUCLEASE-GAMMA;NUCLEASE;NUCLEASE S1 (ASPERGILLUS ORYZAE);NUCLEASE S1;NUCLEASE R;NUCLEASE P1
• CAS NO: 9025-65-4
• Molecular Weight: 0
• EINECS: 253-443-9
• Mol File: 9025-65-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: MUNG BEAN NUCLEASE(CAS DataBase Reference)
• NIST Chemistry Reference: MUNG BEAN NUCLEASE(9025-65-4)
• EPA Substance Registry System: MUNG BEAN NUCLEASE(9025-65-4)

Safety Data

• Safety Statements: 23-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9025-65-4(Hazardous Substances Data)

Usage

Description

MUNG BEAN NUCLEASE, also known as Endonuclease from Serratia marcescens, is a magnesium-dependent nuclease enzyme that forms a dimer with two identical monomeric units and distinct protein folds. Its core structure consists of a six-stranded antiparallel β-sheet flanked by α-helices on either side, with each monomer bearing one active site.

Uses

Used in Molecular Biology Research:
MUNG BEAN NUCLEASE is used as a detection tool for assays to measure the amount of Tf1 RNA protected from degradation by nucleases, such as Benzonase. This helps in studying the properties and behavior of the Tf1 element of Schizosaccharomyces pombe, a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins.
Used in Cell Biology:
MUNG BEAN NUCLEASE is used as a reagent for limiting cell clumping during the preparation of chimeric cell mixtures. It aids in the proper mixing and analysis of different cell types in research studies.
Used in Protein Biochemistry:
MUNG BEAN NUCLEASE is used as a component of lysis buffer for protein extraction from cell lines in affinity purification studies. It helps in the efficient extraction of proteins by digesting DNA and releasing nuclear proteins that are intimately associated with DNA.
Used in Proximity Biotinylation Assays:
MUNG BEAN NUCLEASE, specifically Turbonuclease from Serratia marcescens, is used for cell lysis during proximity biotinylation assays (BioID) and affinity-purification. This allows for the identification and characterization of protein-protein interactions and their spatial organization within cells.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.