9029-33-8

Basic information

• Product Name: EC 1.18.1.2
• Synonyms: EC 1.18.1.2;FERREDOXIN-NADP(+) REDUCTASE;FERRIDOXIN-NADP(+) OXIDO-REDUCTASE;ferredoxin-nadp reductase from spinach*leaves;ferredoxin-nadp+ reductase from spinacia oleracea (spinach)
• CAS NO: 9029-33-8
• Molecular Weight: 0
• Mol File: 9029-33-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• CAS DataBase Reference: EC 1.18.1.2(CAS DataBase Reference)
• NIST Chemistry Reference: EC 1.18.1.2(9029-33-8)
• EPA Substance Registry System: EC 1.18.1.2(9029-33-8)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9029-33-8(Hazardous Substances Data)

Usage

Description

EC 1.18.1.2, also known as Ferredoxin-NADP+ Reductase, is an enzyme that plays a crucial role in the electron transport chain during photosynthesis. It catalyzes the reduction of NADP+ to NADPH, which is then used in various metabolic processes, including the Calvin cycle and fatty acid synthesis. This enzyme is found in various organisms, including plants, algae, and some bacteria.
Used in Plant Biology Research:
EC 1.18.1.2 is used as a research tool for studying the electron transport chain and photosynthesis in plants. It helps researchers understand the mechanisms of energy production and transfer in plant cells.
Used in Enzyme Assays:
Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) is used as a component in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay. This application allows researchers to study the activity and regulation of PcyA enzyme, which is involved in the biosynthesis of phycobilins, essential for photosynthesis in cyanobacteria and red algae.
Used in Testing Protein Interactions:
EC 1.18.1.2 has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)?CP12 preparation. This application helps researchers investigate the interactions between different proteins and their impact on metabolic processes in cells.

Biochem/physiol Actions

Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.