9032-88-6

Basic information

• Product Name: EC 4.2.1.2
• Synonyms: L-MALATE HYDROLYASE;FUMARASE;FUMARATE HYDRATASE;EC 4.2.1.2;fumarase from porcine heart;fumarase from rabbit liver;Hydratase, fumarate;FUMARASE PRESTAINED FROM PORCINE HEART
• CAS NO: 9032-88-6
• Molecular Weight: 0
• EINECS: 232-887-7
• Mol File: 9032-88-6.mol
• Article Data: 0

Chemical Properties

• Appearance: /ammonium sulfate suspension
• Storage Temp.: 2-8°C
• CAS DataBase Reference: EC 4.2.1.2(CAS DataBase Reference)
• NIST Chemistry Reference: EC 4.2.1.2(9032-88-6)
• EPA Substance Registry System: EC 4.2.1.2(9032-88-6)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 9032-88-6(Hazardous Substances Data)

Usage

Description

EC 4.2.1.2, also known as fumarase, is an enzyme that catalyzes the reversible hydration of fumarate to L-malate. It plays a crucial role in the citric acid cycle, also known as the Krebs cycle or the tricarboxylic acid (TCA) cycle, which is a key metabolic pathway for energy production in cells.
Used in Biochemical Research:
EC 4.2.1.2 is used as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assays. This application is important for evaluating the stability and activity of immobilized enzymes, which can be used in various industrial and research applications.
Used in Medical Research:
Fumarase from porcine heart is used in fumarate hydratase assays in human lung carcinoma cells (A549). This application is significant for studying the role of fumarate hydratase in cancer cells and for developing potential therapeutic strategies targeting this enzyme.

Biochem/physiol Actions

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.