9050-94-6

Basic information

• Product Name: SEPHADEX G-100
• Synonyms: Cross-inked dextran gel G-100;Sephadex(R) G-100, medium;Cross-inked dextran gel G-75;Sephadex G-100 100-200;Sephadex G-100 200-400;Sephadex G-100 60-100;SEPHADEX G-100;SEPHADEX(R) G-100
• CAS NO: 9050-94-6
• Molecular Formula: NULL
• Molecular Weight: 0
• Product Categories: Gel Filtration MediaResins&LC Separation Media;Sepharose;Gel Filtration MediaGel Filtration Media;Sephadex G-100;Gel Filtration Chromatography;Protein Chromatography;Sephadex;DNA&RNA Purification;Molecular Biology;Reagents;Gel Filtration Media
• Mol File: 9050-94-6.mol
• Article Data: 0

Chemical Properties

• Flash Point: 38 °C
• Appearance: /
• Storage Temp.: room temp
• CAS DataBase Reference: SEPHADEX G-100(CAS DataBase Reference)
• NIST Chemistry Reference: SEPHADEX G-100(9050-94-6)
• EPA Substance Registry System: SEPHADEX G-100(9050-94-6)

Safety Data

• Statements: 10
• Safety Statements: 22-16
• RIDADR: NA 1993 / PGIII
• WGK Germany: 3
• Hazardous Substances Data: 9050-94-6(Hazardous Substances Data)

Usage

Description

SEPHADEX G-100 is a gel filtration medium used in protein chromatography, affinity chromatography, and gel filtration chromatography. It is a cross-linked agarose gel filtration base matrix that is useful for desalting and buffer exchange of very large biomolecules. It quickly desalts, removes contaminants, and transfers to a new buffer in a single step.
Used in Biochemistry Research:
SEPHADEX G-100 is used as a purification agent for the separation and purification of proteins, peptides, and other biomolecules due to its ability to efficiently remove contaminants such as salts, dyes, and radioactive labels.
Used in Pharmaceutical Industry:
SEPHADEX G-100 is used as a chromatography medium for the purification of drugs and drug candidates, ensuring the purity and quality of the final product.
Used in Clinical Applications:
SEPHADEX G-100 is used as a diagnostic tool in clinical laboratories for the separation and analysis of biological samples, such as proteins and peptides, to aid in the diagnosis and monitoring of various diseases.
Used in Food and Beverage Industry:
SEPHADEX G-100 is used as a purification agent in the food and beverage industry for the separation and purification of proteins, enzymes, and other biomolecules, ensuring the quality and safety of the final product.
Used in Environmental Applications:
SEPHADEX G-100 is used as a purification agent in environmental applications for the separation and purification of pollutants and contaminants from water and soil samples, contributing to environmental protection and remediation efforts.
Used in Cosmetics Industry:
SEPHADEX G-100 is used as a purification agent in the cosmetics industry for the separation and purification of active ingredients, such as peptides and proteins, ensuring the efficacy and safety of cosmetic products.
Used in Agricultural Applications:
SEPHADEX G-100 is used as a purification agent in agricultural applications for the separation and purification of plant proteins, enzymes, and other biomolecules, contributing to the development of sustainable and efficient agricultural practices.

History

The introduction of Sephadex in 1959 heralded a new era in separation science. The concept of gel permeation chromatography (GPC) developed slowly and the key events leading to the discovery of Sephadex may be traced to Ingelman ?s early studies on the cross-linking of dextran, the subsequent patent by Flodin and Ingelman on the preparation of cross-linked gels and the exploitation of their gel filtration properties by Flodin and Porath. Perhaps the most critical event was the synthesis of these gels in bead form with considerably improved flow rates, an innovation that led to the immediate acceptance of these gels in separation technology. Several types of Sephadex are currently available, each with a characteristic fractionation range. The most porous gel, Sephadex G-200, will fractionate proteins in the Mw range 4000–800000, whereas the upper limit for Sephadex G-25 is 5000. Although Sephadex gels are widely used for fractionation and purification of biopolymers, desalting constitutes one of the most important applications. In this context, desalting also refers to buffer exchange operations and the removal of low molecular weight impurities other than salts. Sephadex G-25 is now used for desalting or buffer exchange in many large scale operations. Insulin producers use Sephadex G-50 to remove proinsulin and protease impurities in the final stages of purification of porcine or bovine insulins. The structure of Sephadex G-25 has recently been investigated by Holmberg (285) and the following novel structural features have come to light. Only 4% of the glucose units are cross-linked. Eight different structural elements were identified, including several glyceryl ethers of glucose and cyclic structures containing 1,4-dioxane rings. Two of these fragments are represented below 3 and 4. 80% of the beads consists of dextran.