94736-09-1

Basic information

• Product Name: DUTP, 100MM SOLUTION
• Synonyms: DUTP, 100MM SOLUTION;2'-deoxyuridine 5'-triphosphate sodium salt solution;2μ-Deoxyuridine 5μ-triphosphate solution sodium salt
• CAS NO: 94736-09-1
• Molecular Formula: C9H15N2O14P3
• Molecular Weight: 492.14
• Mol File: 94736-09-1.mol
• Article Data: 0

Chemical Properties

• Appearance: colorless/
• Storage Temp.: −20°C
• CAS DataBase Reference: DUTP, 100MM SOLUTION(CAS DataBase Reference)
• NIST Chemistry Reference: DUTP, 100MM SOLUTION(94736-09-1)
• EPA Substance Registry System: DUTP, 100MM SOLUTION(94736-09-1)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 94736-09-1(Hazardous Substances Data)

Usage

Description

dUTP, 100mM Solution, also known as deoxyuridine triphosphate, is a nucleotide that is structurally similar to dTTP (deoxythymidine triphosphate) but contains the base uracil instead of thymine. It is a PCR-grade, 100 mM clear, colorless solution of the sodium salt with a pH of 8.3. This high-quality reagent is designed to minimize carryover contamination between PCRs, thus reducing the risk of false positives.

Uses

Used in Molecular Biology Research:
dUTP, 100mM Solution is used as a substitute for dTTP in various molecular biology applications, such as PCR, to prevent carryover contamination and false positives. The presence of uracil instead of thymine in dUTP makes it a useful alternative for sensitive applications where contamination control is crucial.
Used in Uracil-N-Glycosylase (UNG) PCR:
dUTP, 100mM Solution is used as a component in UNG PCR, a technique that incorporates uracil into the amplified DNA during the PCR process. The subsequent treatment with uracil-N-glycosylase (UNG) enzyme cleaves the DNA at the sites of uracil incorporation, effectively destroying any carryover contamination from previous PCR reactions.
Used in DNA Fingerprinting and Genotyping:
dUTP, 100mM Solution is used as a high-quality reagent in DNA fingerprinting and genotyping applications, where accurate and reliable results are essential. Its ability to minimize contamination makes it a preferred choice for these sensitive techniques.
Used in Diagnostics and Forensic Analysis:
In diagnostics and forensic analysis, dUTP, 100mM Solution is used to ensure the accuracy and reliability of DNA-based tests. Its role in preventing carryover contamination makes it a valuable tool in these fields, where the consequences of false positives can be significant.
Used in Synthetic Biology and DNA Assembly:
dUTP, 100mM Solution is used in synthetic biology and DNA assembly processes, where the creation of specific DNA sequences or constructs is required. Its high purity and quality make it suitable for these applications, where precise manipulation of genetic material is necessary.