95740-26-4 Usage
Description
(2'S)-2'-Deoxy-2'-fluoro-5-ethynyluridine is a nucleoside analog that is commonly used as a probe for DNA synthesis in vivo. It is characterized by its minimal impact on genome function across various tissue types and is particularly useful for metabolic DNA labeling when prolonged cell survival and undisturbed cell cycle progression are necessary.
Uses
Used in Biomedical Research:
(2'S)-2'-Deoxy-2'-fluoro-5-ethynyluridine is used as a probe for DNA synthesis in vivo for its less toxic nature compared to EdU and BrdU. It is particularly useful for metabolic DNA labeling when prolonged cell survival and undisturbed cell cycle progression are required, such as in pulse-chase experiments aimed at "birth dating" DNA in vivo.
Used in Cellular Biology Studies:
In cellular biology, (2'S)-2'-Deoxy-2'-fluoro-5-ethynyluridine serves as a valuable tool for tracking DNA synthesis and cell proliferation. Its minimal impact on cell function makes it an ideal choice for studies where the integrity of the cell cycle and cell survival are crucial for accurate results.
Check Digit Verification of cas no
The CAS Registry Mumber 95740-26-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,5,7,4 and 0 respectively; the second part has 2 digits, 2 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 95740-26:
(7*9)+(6*5)+(5*7)+(4*4)+(3*0)+(2*2)+(1*6)=154
154 % 10 = 4
So 95740-26-4 is a valid CAS Registry Number.
InChI:InChI=1/C11H11FN2O5/c1-2-5-3-14(11(18)13-9(5)17)10-7(12)8(16)6(4-15)19-10/h1,3,6-8,10,15-16H,4H2,(H,13,17,18)/t6-,7+,8-,10-/m1/s1
95740-26-4Relevant articles and documents
Compatibility of 5-ethynyl-2′F-ANA UTP with: In vitro selection for the generation of base-modified, nuclease resistant aptamers
Levi-Acobas, Fabienne,Katolik, Adam,R?thlisberger, Pascal,Cokelaer, Thomas,Sarac, Ivo,Damha, Masad J.,Leumann, Christian J.,Hollenstein, Marcel
, p. 8083 - 8087 (2019)
A modified nucleoside triphosphate bearing two modifications based on a 2′-deoxy-2′-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2′F-ANA UTP) was synthesized. This nucleotide analog could enzymatically
