96801-39-7

Basic information

• Product Name: 12-(7-NITROBENZOFURAZAN-4-YLAMINO)DODECANOIC ACID
• Synonyms: 12-(7-NITRO-2,1,3-BENZOXADIAZOL-4-YLAMINO)DODECANOIC ACID;12-(7-NITROBENZOFURAZAN-4-YLAMINO)DODECANOIC ACID;12-(N-(7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL)AMINO)DODECANOIC ACID;C12-NBD;C12-NBD,freeacid(12-97-nITROBENZOFURAZAN4-YLAMINO)DODECANOICACID);NBD-dodecanoic acid;NBD-C12 acid [12-(7-Nitrobenzofurazan-4-ylaMino)dodecanoic acid];Dodecanoic acid, 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-
• CAS NO: 96801-39-7
• Molecular Formula: C₁₈H₂₆N₄O₅
• Molecular Weight: 378.42
• Mol File: 96801-39-7.mol
• Article Data: 7

Chemical Properties

• Melting Point: 79-81 °C(lit.)
• Boiling Point: 582.6 °C at 760 mmHg
• Flash Point: 306.2 °C
• Appearance: /
• Density: 1.26 g/cm³
• Vapor Pressure: 2.04E-14mmHg at 25°C
• Refractive Index: 1.589
• Storage Temp.: 2-8°C
• Solubility: DMF: soluble
• BRN: 7392043
• CAS DataBase Reference: 12-(7-NITROBENZOFURAZAN-4-YLAMINO)DODECANOIC ACID(CAS DataBase Reference)
• NIST Chemistry Reference: 12-(7-NITROBENZOFURAZAN-4-YLAMINO)DODECANOIC ACID(96801-39-7)
• EPA Substance Registry System: 12-(7-NITROBENZOFURAZAN-4-YLAMINO)DODECANOIC ACID(96801-39-7)

Safety Data

• WGK Germany: 3
• F: 8-10
• Hazardous Substances Data: 96801-39-7(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 96801-39-7 differently. You can refer to the following data:
1. Suitable for probing the ligand binding sites of fatty acid and sterol carrier proteins as well as the investigation of lipolysis processes.
2. 12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid is an amine-reactive, fluorescent fatty acid analog.

InChI:InChI=1/C18H26N4O5/c23-16(24)10-8-6-4-2-1-3-5-7-9-13-19-14-11-12-15(22(25)26)18-17(14)20-27-21-18/h11-12,19H,1-10,13H2,(H,23,24)

Upstream product

• 53005-24-6 methyl 12-aminododecanoate 
• 110544-78-0 methyl 12-azidododecanoate 
• 26825-95-6 methyl 12-bromododecanoate 
• 10199-89-0 NBD chloride 
• 693-57-2 12-Aminododecanoic acid 

Relevant articles and documents

Identification and Characterization of a Single High-Affinity Fatty Acid Binding Site in Human Serum Albumin

A single high-affinity fatty acid binding site in the important human transport protein serum albumin (HSA) is identified and characterized using an NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-C12 fatty acid. This ligand exhibits a 1:1 binding stoichiometry in its HSA complex with high site-specificity. The complex dissociation constant is determined by titration experiments as well as radioactive equilibrium dialysis. Competition experiments with the known HSA-binding drugs warfarin and ibuprofen confirm the new binding site to be different from Sudlow-sites I and II. These binding studies are extended to other albumin binders and fatty acid derivatives. Furthermore an X-ray crystal structure allows locating the binding site in HSA subdomain IIA. The knowledge about this novel HSA site will be important for drug depot development and for understanding drug-protein interaction, which are important prerequisites for modulation of drug pharmacokinetics.

Development of a novel FRET probe for the real-time determination of ceramidase activity

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Novel fluorescent ceramide derivatives for probing ceramidase substrate specificity

Ceramidases are key regulators of cell fate. The biochemistry of different ceramidases and of their substrate ceramide appears to be complex, mainly due to specific biophysical characteristics at the water-membrane interface. In the present study, we describe the design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases. For the first time we have replaced the commonly used polar NBD-dye with the lipophilic Nile Red (NR) dye. Analysis of kinetic data reveal that although both the dyes do not have any noticeable preference for the substitution at acyl or sphingosine (Sph) part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase.