97753-82-7

Basic information

• Product Name: 5-Bromo-3-indolyl-beta-D-galactopyranoside
• Synonyms: beta-d-galactopyranoside,5-bromo-1h-indol-3-yl;5-BROMOINDOLYL B-D-GALACTOPYRANOSIDEMOLE CULAR BIOL;5-BROMO-3-INDOLYL-B-D-GALACTOSIDE;5-Bromo-3-indolyl-b-D-galactopyranoside;5-bromo-3-indolyl-beta-galactoside;5-BROMO-3-INDOLYL-BETA-D -GALACTOPYRANOSIDE 98+% -20 C;5-BROMO-3-INDOLYL-BETA-D -GALACTOPYRANOSIDE MOLECULAR BIOLOGY GRADE 98+% -20 C;BLUE GAL (5-BROMO-3-INDOLYL- BETA-D-GALACTOPYRANOSIDE)
• CAS NO: 97753-82-7
• Molecular Formula: C14H16BrNO6
• Molecular Weight: 374.18
• EINECS: 1533716-785-6
• Product Categories: Indoles and derivatives;substrate
• Mol File: 97753-82-7.mol
• Article Data: 0

Chemical Properties

• Boiling Point: 646.6oC at 760 mmHg
• Flash Point: 344.9oC
• Appearance: White/Powder
• Density: 1.824g/cm3
• Vapor Pressure: 1.33E-17mmHg at 25°C
• Refractive Index: 1.73
• Storage Temp.: 2-8°C
• Solubility: DMF: soluble
• Water Solubility: Soluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution).
• BRN: 1550140
• CAS DataBase Reference: 5-Bromo-3-indolyl-beta-D-galactopyranoside(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Bromo-3-indolyl-beta-D-galactopyranoside(97753-82-7)
• EPA Substance Registry System: 5-Bromo-3-indolyl-beta-D-galactopyranoside(97753-82-7)

Safety Data

• Hazard Codes: Xn
• Statements: 20/21/22-36/37/38
• Safety Statements: 22-24/25-36-26
• WGK Germany: 3
• Hazardous Substances Data: 97753-82-7(Hazardous Substances Data)

Usage

Description

5-Bromo-3-indolyl-beta-D-galactopyranoside, also known as X-GAL, is a synthetic compound that serves as a substrate for beta-galactosidase enzyme. It is widely used in molecular biology and microbiology for the detection and analysis of specific genes and enzymatic activities.

Uses

Used in Molecular Biology:
5-Bromo-3-indolyl-beta-D-galactopyranoside is used as a substrate for beta-galactosidase in Lac gene detection systems. When the enzyme acts on X-GAL, it is converted into an insoluble indigo-blue chromophore, allowing for the visual identification of gene expression and enzymatic activity.
Used in Microbiology:
5-Bromo-3-indolyl-beta-D-galactopyranoside is used as an α-galactosidase substrate for the identification of lac-(+) bacterial colonies. The conversion of X-GAL to the indigo-blue chromophore provides a visual indication of the presence of the lac+ gene, aiding in the selection and analysis of bacterial strains.
Used in Immunoblotting and Immunocytochemical Assays:
5-Bromo-3-indolyl-beta-D-galactopyranoside is used as a chromogenic substrate in immunoblotting and immunocytochemical assays. The enzyme-linked detection of specific proteins or cellular structures results in the formation of the indigo-blue chromophore, enabling the visualization and analysis of target molecules within biological samples.
Used in Histological Applications:
5-Bromo-3-indolyl-beta-D-galactopyranoside is also employed in histological applications for the detection and localization of specific enzymatic activities within tissue sections. The conversion of X-GAL to the indigo-blue chromophore allows for the visualization of enzyme distribution and activity patterns within various tissue types.

InChI:InChI=1/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1