98516-76-8 Usage
Description
H-GLU-AMC, also known as 7-amino-4-methylcoumarin, is a synthetic fluorogenic substrate commonly used in biochemical assays. It is designed to mimic the structure of peptide bonds and is specifically cleaved by aminopeptidase enzymes. Upon enzymatic cleavage, H-GLU-AMC releases a blue fluorescent product, 7-amino-4-methylcoumarin, which can be detected and quantified using fluorescence spectroscopy.
Uses
Used in Biochemical Research:
H-GLU-AMC is used as a fluorogenic substrate for aminopeptidase enzymes, allowing researchers to study the activity, specificity, and regulation of these enzymes in various biological systems. The blue fluorescent product generated upon cleavage provides a sensitive and quantitative measure of enzymatic activity, facilitating the investigation of enzyme kinetics, inhibition, and substrate preferences.
Used in Drug Discovery and Development:
In the pharmaceutical industry, H-GLU-AMC serves as a valuable tool for the identification and optimization of potential inhibitors or activators of aminopeptidase enzymes. By monitoring the fluorescence generated upon substrate cleavage, researchers can screen large libraries of compounds for their ability to modulate enzyme activity, leading to the discovery of novel therapeutic agents with potential applications in various diseases.
Used in Diagnostic Assays:
H-GLU-AMC can be employed in the development of diagnostic assays to detect and quantify aminopeptidase activity in clinical samples. This can be particularly useful in the diagnosis and monitoring of certain diseases or conditions where aminopeptidase levels may be altered, providing valuable information for patient management and treatment decisions.
Check Digit Verification of cas no
The CAS Registry Mumber 98516-76-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,8,5,1 and 6 respectively; the second part has 2 digits, 7 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 98516-76:
(7*9)+(6*8)+(5*5)+(4*1)+(3*6)+(2*7)+(1*6)=178
178 % 10 = 8
So 98516-76-8 is a valid CAS Registry Number.
InChI:InChI=1/C15H16N2O5/c1-8-6-14(20)22-12-7-9(2-3-10(8)12)17-15(21)11(16)4-5-13(18)19/h2-3,6-7,11H,4-5,16H2,1H3,(H,17,21)(H,18,19)/t11-/m0/s1
