99792-79-7 Usage
Description
Chlorophenol red β-D-galactopyranoside (CPRG) is a colorimetric substrate specifically designed for the detection and quantification of β-galactosidase activity. CHLOROPHENOL RED-BETA-D-GALACTOPYRANOSID serves as an indicator of enzymatic activity, allowing for the measurement of β-galactosidase expression levels in various applications.
Uses
Used in Molecular Biology and Biotechnology:
Chlorophenol Red-β-D-galactopyranoside is used as a substrate for β-galactosidase to measure the expression levels of the enzyme in transfected cells. This application is crucial for studying gene expression and the functionality of recombinant proteins in various biological systems.
Used in Diagnostics and Clinical Research:
In the field of diagnostics and clinical research, Chlorophenol Red-β-D-galactopyranoside serves as a colorimetric substrate for the detection of multiple clinically relevant mutations using a lacZ output gene. This allows for the identification and analysis of genetic variations that may have implications for disease progression or treatment response.
Used in Microbiology:
Chlorophenol Red-β-D-galactopyranoside is utilized as a substrate for β-galactosidase in colorimetric assays for bacteria quantification. This application aids in the assessment of bacterial growth and the study of bacterial metabolism, which is essential for understanding microbial dynamics in various environments.
Used in Paper-based Cell-free Protein Expression Systems:
Chlorophenol Red-β-D-galactopyranoside has been employed as a substrate in paper-based cell-free protein expression systems for target RNA detection. This innovative approach allows for the detection of specific RNA sequences, which can be valuable in molecular diagnostics and the study of gene regulation.
Used in Colorimetric Determination and ELISA:
Chlorophenol Red-β-D-galactopyranoside is used in colorimetric determination of β-Gal activity in cell extracts and enzyme-linked immunosorbent assays (ELISA). These techniques are widely used in research and clinical settings to quantify enzyme activity and analyze protein-protein interactions, contributing to a better understanding of biological processes and disease mechanisms.
Biochem/physiol Actions
Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.
Check Digit Verification of cas no
The CAS Registry Mumber 99792-79-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,9,7,9 and 2 respectively; the second part has 2 digits, 7 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 99792-79:
(7*9)+(6*9)+(5*7)+(4*9)+(3*2)+(2*7)+(1*9)=217
217 % 10 = 7
So 99792-79-7 is a valid CAS Registry Number.
InChI:InChI=1/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)25(14-3-1-2-4-20(14)38(33,34)37-25)13-6-8-18(16(27)10-13)35-24-23(32)22(31)21(30)19(11-28)36-24/h1-10,19,21-24,28-32H,11H2/t19-,21+,22+,23-,24-,25?/m1/s1