Diethyl pyrocarbonate (cas 1609-47-8) does not degrade RNA

Add time:07/27/2019    Source:sciencedirect.com

SummaryDiethyl pyrocarbonate (cas 1609-47-8), a compound extensively used as a nuclease inhibitor, reacts with low molecular weight RNA and forms products which are less precipitable with certain precipitating reagents than untreated RNA. This phenomenon, erroneously interpreted by Wiegers and Hilz (Biochem. Biophys. Res. Commun. 44 (1971) 513) as degradation of RNA by diethyl pyrocarbonate, is now shown to be due to increased solubility of the treated low molecular weight RNA in precipitating reagents. This conclusion is based on (a) unaltered sedimentation pattern, (b) unchanged content in dialyzable fragments and (c) identical chain length of diethyl pyrocarbonate-treated RNA as compared to the untreated control.

We also recommend Trading Suppliers and Manufacturers of Diethyl pyrocarbonate (cas 1609-47-8). Pls Click Website Link as below: cas 1609-47-8 suppliers

Prev:Diethyl pyrocarbonate (cas 1609-47-8) inactivates CD39/ecto-ATPDase by modifying His-59
Next:Diethyl pyrocarbonate (cas 1609-47-8) (DEPC) inhibits CO2 chemosensitivity in Helix aspersa)