Mica (cas 12001-26-2)-129 A/G dimorphism, its relation to soluble Mica (cas 12001-26-2) plasma level and spontaneous preterm birth: A case-control study
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Add time:07/24/2019 Source:sciencedirect.com
The aim of this case- control study was to investigate the association between preterm birth (PTB), Mica (cas 12001-26-2)-129 A/G dimorphism and sMICA levels. Fifty pregnant women with singleton pregnancy and previous PTB, or clinic diagnostic of threatened preterm labor in the actual pregnancy, or cervical length less than 25 mm and 50 healthy pregnant women were enrolled. DNA was extracted for genotyping for MICA-129 A/G by real-time PCR and sMICA plasma level was quantified by sandwich ELISA assay. Clinical and socioeconomic characteristics, results of TaqMan® genotyping and ELISA quantification were compared between the groups using qui-square, Fisher´s exact or Mann-Whitney test. A binary logistic regression model was used to predict PTB. The correlation between MICA-129 A/G genotypes and sMICA levels was investigated. There were not statistically significant differences between MICA-129 A/G polymorphism and sMICA plasma level.There was found a correlation between MICA-129 val/val genotype and higher levels of sMICA (ρ: -0.342; p:0.001). The presence of MICA-129 val/val genotype may be influencing sMICA expression.
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