Determination of methylphenidate and its metabolite Ritalinic acid (cas 19395-41-6) in urine by liquid chromatography/tandem mass spectrometry
-
Add time:08/18/2019 Source:sciencedirect.com
Methylphenidate (MPH) is a drug that is licensed for treatment of ADHD and also narcolepsy. Monitoring of the parent drug and its major metabolite Ritalinic acid (cas 19395-41-6) (RA) in urine is considered necessary to ensure compliance with treatment programmes. A rapid, simple and sensitive liquid chromatography/tandem mass spectrometry (LC–MS/MS) assay was developed for the determination of MPH and its metabolite RA in human urine. After urine was diluted with water, methylphenidate, the major metabolite ritalinic acid, and d6-amphetamine as the internal standard were resolved on a PFP propyl column using gradient elution of 0.02% ammonium formate and acetonitrile. The total analysis time was 13.5 min. The three compounds were detected using electrospray ionisation in the positive mode. Standard curves were linear over the concentration range 5–5000 μg/L (r > 0.997), bias was ≤±20%, intra- and inter-day coefficients of variation (imprecision) were <8% and the limit of detection was 5 μg/L. The limit of quantitation was set at 100 μg/L. Matrix effects were up to 140% but these were accounted for by the internal standard. The assay is being used successfully in clinical practice to enhance the safe and effective use of methylphenidate.
We also recommend Trading Suppliers and Manufacturers of Ritalinic acid (cas 19395-41-6). Pls Click Website Link as below: cas 19395-41-6 suppliers
Prev:Occurrence and fate of the human pharmaceutical metabolite Ritalinic acid (cas 19395-41-6) in the aquatic system
Next:Potential trends in Attention Deficit Hyperactivity Disorder (ADHD) drug use on a college campus: Wastewater analysis of amphetamine and Ritalinic acid (cas 19395-41-6)) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Determination of methylphenidate and Ritalinic acid (cas 19395-41-6) in blood, plasma and oral fluid from adolescents and adults using protein precipitation and liquid chromatography tandem mass spectrometry—A method applied on clinical and forensic investigations08/20/2019
- Potential trends in Attention Deficit Hyperactivity Disorder (ADHD) drug use on a college campus: Wastewater analysis of amphetamine and Ritalinic acid (cas 19395-41-6)08/19/2019
- Occurrence and fate of the human pharmaceutical metabolite Ritalinic acid (cas 19395-41-6) in the aquatic system08/17/2019
- Rapid quantitative analysis of methylphenidate and Ritalinic acid (cas 19395-41-6) in oral fluid by liquid chromatography triple quadrupole mass spectrometry (LC-QqQ-MS)08/16/2019
- Simultaneous determination of methylphenidate and Ritalinic acid (cas 19395-41-6) in hair using LC–MS/MS08/15/2019
- Full length ArticleBacterial isolates degrading Ritalinic acid (cas 19395-41-6)—human metabolite of neuro enhancer methylphenidate08/14/2019
- Full length ArticleBiotransformation of Ritalinic acid (cas 19395-41-6) by laccase in the presence of mediator TEMPO08/13/2019
-
Health and Chemical more >