Simultaneous determination of sodium iron Chlorophyllin (cas 11006-34-1) and sodium copper Chlorophyllin (cas 11006-34-1) in food using high-performance liquid chromatography and ultra-performance liquid chromatography–mass spectrometry

Add time:08/14/2019    Source:sciencedirect.com

A simultaneous method for analyzing sodium iron Chlorophyllin (cas 11006-34-1) (SIC) and sodium copper chlorophyllin (SCC) using high-performance liquid chromatography was developed. This method employed an Inertsil ODS-2 column and diode array detection at 395 nm, using methanol–water (97:3 and 80:20, v/v) containing 1% acetic acid as the mobile phase. Liquid chromatography–tandem mass spectrometry was used to identify the main components of SIC and SCC as Fe-isochlorine e4 and Cu-isochlorine e4, respectively. The limits of detection and quantitation of SIC were 1.2 and 4.1 mg/kg, respectively, while those of SCC were 1.4 and 4.8 mg/kg, respectively. For intraday and interday tests, the SIC recoveries from candy ranged from 81% to 101%, while SCC recoveries ranged from 100% to 109%. The developed method can be applied to the rapid determination of SIC and SCC in candy.

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