Short communicationDetermination of Avilamycin (cas 11051-71-1) A and B in pig faeces by solid phase extraction and reverse-phase HPLC assay
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Add time:08/19/2019 Source:sciencedirect.com
A HPLC method is described for the simultaneous determination of Avilamycin (cas 11051-71-1) A and B in pig faeces, following extraction using acetonitrile and normal-phase solid phase extraction. The HPLC stationary phase was Kromosil 5 μ C-18 with a mobile phase of 48% acetonitrile and 52% 0.01N ammonium acetate buffer, pumped at a flow rate of 1 ml/min. Detection was by UV absorbance at 295 nm and an injection volume of 50 μl was used. Recovery from faeces was >98% and intra-assay precision (CV) was <9.0% for both compounds. The lowest limit of quantification was 0.9 mg/kg (avilamycin A) and 0.2 mg/kg (avilamycin B) with an accuracy of <15% error. No interference was seen from endogenous materials in pig faeces and commonly used veterinary antibiotics.
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