PINK1/Parkin-mediated mitophagy was activated against 1,4-Benzoquinone (cas 106-51-4)-induced apoptosis in HL-60 cells
-
Add time:08/20/2019 Source:sciencedirect.com
Hematotoxicity of benzene is derived mainly from its active metabolite, 1,4-Benzoquinone (1,4-BQ), which induces cell apoptosis and mitochondrial damage. Damaged mitochondria are degraded through a specialized autophagy pathway, called mitophagy, which is driven by PINK1/Parkin signaling. However, whether mitophagy is involved in 1,4-BQ-induced toxicity remains unclear. This study was designed to investigate whether PINK1/Parkin-mediated mitophagy is activated in 1,4-BQ-treated HL-60 cells, and the roles mitophagy plays in 1,4-BQ-induced apoptosis. Our results demonstrated that 1,4-BQ induced autophagy in HL-60 cells, characterized by increased LC3-II/LC3-I ratio and Beclin1 expression, as well as decreased expression of p62. We confirmed the presence of mitophagosomes using electron microscopy, and found that 1,4-BQ-induced autophagy was blocked by pretreatment with the mitophagy inhibitor Cyclosporine A (CsA). In addition, we found that 1,4-BQ induced mitochondrial stress through decreased mitochondrial membrane potential (MMP) and increasedproduction of reactive oxygen species (ROS). We also confirmed that 1,4-BQ-induced mitophagy was mediated by the PINK1/Parkin pathway, illustrated by increased expression of PINK1 and Parkin mRNA and protein. Finally, we examined 1,4-BQ-induced apoptosis with or without CsA, which demonstrated that apoptosis increased after mitophagy inhibition, suggesting that mitophagy has a protective effect in this context. In conclusion, this study demonstrates that the activated PINK1/Parkin-mediated mitophagy exerts a significantly protective effect against 1,4-BQ-induced apoptosis in HL-60 cells.
We also recommend Trading Suppliers and Manufacturers of 1,4-Benzoquinone (cas 106-51-4). Pls Click Website Link as below: cas 106-51-4 suppliers
Prev:Photochemical processes of 1,4-Benzoquinone (cas 106-51-4)s in aqueous medium
Next:Synthesis of 2-substituted hydroquinone derivatives from 1,4-Benzoquinone (cas 106-51-4) and allyl ethers) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Core-shell structured 1,[email protected]2 cathode for lithium batteries08/27/2019
- Theoretical study of the structure and specific capacity of an organic cathode based on poly(2,5-diaza-1,4-Benzoquinone (cas 106-51-4)) in a lithiated state08/26/2019
- Synthesis of a polyacrylonitrile/tetrachloro-1,4-Benzoquinone (cas 106-51-4) gel polymer electrolyte for high-performance Li-air batteries08/25/2019
- Original articleSpectroscopic studies on the formation of charge transfer complexes of l-phenylalanine with 2,3,5-trichloro-6-alkoxy-1,4-Benzoquinone (cas 106-51-4)s in aqueous medium08/24/2019
- Highly stable 2,3,5,6-tetrachloro-1,4-Benzoquinone (cas 106-51-4) electrodes for supercapacitors08/23/2019
- UV-induced transformation of 2,3-dibromo-5,6-dimethyl-1,4-Benzoquinone (cas 106-51-4) in water and treated wastewater08/22/2019
- Synthesis of 2-substituted hydroquinone derivatives from 1,4-Benzoquinone (cas 106-51-4) and allyl ethers08/21/2019
- Photochemical processes of 1,4-Benzoquinone (cas 106-51-4)s in aqueous medium08/19/2019
- Research paperThe evaluation of 1,4-Benzoquinone (cas 106-51-4)s as inhibitors of human monoamine oxidase08/18/2019