Research articleIsolation and functional analysis of Squalene (cas 111-02-4) synthase gene in tea plant Camellia sinensis
-
Add time:08/22/2019 Source:sciencedirect.com
Tea contains high quantities and diverse types of triterpenoids, particularly in the form of saponins. However, little is yet known about the molecular basis of triterpenoid biosynthesis in tea plant. Here we report on isolation and functional analysis of Squalene (cas 111-02-4) synthase (SQS) gene from tea plant (Camellia sinensis var. sinensis), which controls the biosynthesis of triterpenoids precursor. First, a full-length cDNA of squalene synthase, designated CsSQS, was isolated from tea plant. The protein is highly homologous to SQSs from other plants. Using CsSQS-reporter assays, CsSQS was demonstrated to be endoplasmic reticulum membrane-bound. The coding region of CsSQS excluding transmemberane sequence was expressed in Escherichia coli. Recombinant CsSQS catalyzed the formation of squalene using farnesyl-pyrophosphate (FPP) as substrate with NADPH and Mg2+. In tea plant leaves, CsSQS expression was significantly induced by both herbivore and mechanical damages. Consistent with the stronger induction of CsSQS expression by mechanical damage than herbivory, tea plants injured mechanically released squalene as a volatile compound, which however was not detected from herbivore-damaged tea plants. Furthermore, it was found that the flowers of another tea plant cultivar Camellia sinensis var. assamica contain higher concentrations of squalene than the cultivar sinensis, indicating variations among tea plant varieties. With the identification and molecular characterization of squalene synthase in tea plant, next, we can ask the questions about the roles of squalene as a volatile product as well as a precursor for triterpenoids, which may promote product development from diverse tea materials and mining of excellent tea germplasm resources.
We also recommend Trading Suppliers and Manufacturers of Squalene (cas 111-02-4). Pls Click Website Link as below: cas 111-02-4 suppliers
Prev:Regular articleSystems-based Saccharomyces cerevisiae strain design for improved Squalene (cas 111-02-4) synthesis
Next:Chitosan – Whey protein as efficient delivery system for Squalene (cas 111-02-4): Characterization and functional food application) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Production of Squalene (cas 111-02-4) with promising antioxidant properties in callus cultures of Nilgirianthus ciliatus08/28/2019
- Short communicationSimple and rapid method to analyse Squalene (cas 111-02-4) in olive oils and extra virgin olive oils08/27/2019
- Biophysical characterization of asolectin-Squalene (cas 111-02-4) liposomes08/26/2019
- Translation of nanomedicines from lab to industrial scale synthesis: The case of Squalene (cas 111-02-4)-adenosine nanoparticles08/25/2019
- Method ArticleDetermination of Squalene (cas 111-02-4) in edible oils by transmethylation and GC analysis08/24/2019
- Chitosan – Whey protein as efficient delivery system for Squalene (cas 111-02-4): Characterization and functional food application08/23/2019
- Regular articleSystems-based Saccharomyces cerevisiae strain design for improved Squalene (cas 111-02-4) synthesis08/21/2019
- Research paper1H NMR combined with PLS for the rapid determination of Squalene (cas 111-02-4) and sterols in vegetable oils08/20/2019